Effect of hesperidin on anti-inflammation and cellular antioxidant capacity in hydrogen peroxide-stimulated human articular chondrocytes

Yuh Feng Tsai, Yi Ru Chen, Jo Ping Chen, Yun Tang, Kai Chiang Yang

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

Excessive levels of oxidative stress can induce a senescence-associated secretory phenotype in chondrocytes which is characterized by the secretions of inflammatory mediators. Citrus flavonoid hesperidin (Hsd) is known to have anti-inflammatory and antioxidant capacities. Accordingly, we demonstrated the antioxidant property of Hsd against hydrogen peroxide (H2O2)-induced oxidative stress damages to chondrocytes in this study. Primary human chondrocytes were cultured in media supplemented with Hsd (5, 10, 50 and 100 μM) to evaluate the dose-dependent cytotoxicity. Subsequently, cells were stimulated by H2O2 and supplied with Hsd to study chondroprotective effects. Results showed that high-dose of Hsd (50 and 100 μM) has a deleterious effect on normal cells, whilst low Hsd (5 and 10 μM) supplements improved mitochondrial activity, cytotoxicity, proliferation kinetic, survival and cell senescence in H2O2-stimulated chondrocytes. Real-time PCR revealed that Hsd downregulated the mRNA levels of COX-2, IL-1β, TNF-α, MMP-3, MMP-9, and upregulated IL-10, TIMP-1, SOX9. Western blotting further revealed that Hsd modulates Foxo1, Foxo3 and Nrf2 signaling pathways. Furthermore, Hsd improved total antioxidant capacity, and restored superoxide dismutases and glutathione peroxidase activities in H2O2-stimulated chondrocytes. In conclusion, our findings suggest that Hsd has the potential to improve antioxidant capacity in human chondrocytes.

原文英語
期刊Process Biochemistry
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

Hesperidin
Chondrocytes
Antioxidants
Hydrogen peroxide
Hydrogen Peroxide
Joints
Inflammation
Oxidative stress
Cytotoxicity
Flavonoids
Matrix Metalloproteinases
Oxidative Stress
Kinetics
Far-Western Blotting
Tissue Inhibitor of Metalloproteinase-1
Citrus
Cell Aging
Glutathione Peroxidase
Interleukin-1
Interleukin-10

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

引用此文

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title = "Effect of hesperidin on anti-inflammation and cellular antioxidant capacity in hydrogen peroxide-stimulated human articular chondrocytes",
abstract = "Excessive levels of oxidative stress can induce a senescence-associated secretory phenotype in chondrocytes which is characterized by the secretions of inflammatory mediators. Citrus flavonoid hesperidin (Hsd) is known to have anti-inflammatory and antioxidant capacities. Accordingly, we demonstrated the antioxidant property of Hsd against hydrogen peroxide (H2O2)-induced oxidative stress damages to chondrocytes in this study. Primary human chondrocytes were cultured in media supplemented with Hsd (5, 10, 50 and 100 μM) to evaluate the dose-dependent cytotoxicity. Subsequently, cells were stimulated by H2O2 and supplied with Hsd to study chondroprotective effects. Results showed that high-dose of Hsd (50 and 100 μM) has a deleterious effect on normal cells, whilst low Hsd (5 and 10 μM) supplements improved mitochondrial activity, cytotoxicity, proliferation kinetic, survival and cell senescence in H2O2-stimulated chondrocytes. Real-time PCR revealed that Hsd downregulated the mRNA levels of COX-2, IL-1β, TNF-α, MMP-3, MMP-9, and upregulated IL-10, TIMP-1, SOX9. Western blotting further revealed that Hsd modulates Foxo1, Foxo3 and Nrf2 signaling pathways. Furthermore, Hsd improved total antioxidant capacity, and restored superoxide dismutases and glutathione peroxidase activities in H2O2-stimulated chondrocytes. In conclusion, our findings suggest that Hsd has the potential to improve antioxidant capacity in human chondrocytes.",
keywords = "Antioxidant, Hesperidin, Inflammation, Osteoarthritis, Oxidative stress",
author = "Tsai, {Yuh Feng} and Chen, {Yi Ru} and Chen, {Jo Ping} and Yun Tang and Yang, {Kai Chiang}",
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T1 - Effect of hesperidin on anti-inflammation and cellular antioxidant capacity in hydrogen peroxide-stimulated human articular chondrocytes

AU - Tsai, Yuh Feng

AU - Chen, Yi Ru

AU - Chen, Jo Ping

AU - Tang, Yun

AU - Yang, Kai Chiang

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Excessive levels of oxidative stress can induce a senescence-associated secretory phenotype in chondrocytes which is characterized by the secretions of inflammatory mediators. Citrus flavonoid hesperidin (Hsd) is known to have anti-inflammatory and antioxidant capacities. Accordingly, we demonstrated the antioxidant property of Hsd against hydrogen peroxide (H2O2)-induced oxidative stress damages to chondrocytes in this study. Primary human chondrocytes were cultured in media supplemented with Hsd (5, 10, 50 and 100 μM) to evaluate the dose-dependent cytotoxicity. Subsequently, cells were stimulated by H2O2 and supplied with Hsd to study chondroprotective effects. Results showed that high-dose of Hsd (50 and 100 μM) has a deleterious effect on normal cells, whilst low Hsd (5 and 10 μM) supplements improved mitochondrial activity, cytotoxicity, proliferation kinetic, survival and cell senescence in H2O2-stimulated chondrocytes. Real-time PCR revealed that Hsd downregulated the mRNA levels of COX-2, IL-1β, TNF-α, MMP-3, MMP-9, and upregulated IL-10, TIMP-1, SOX9. Western blotting further revealed that Hsd modulates Foxo1, Foxo3 and Nrf2 signaling pathways. Furthermore, Hsd improved total antioxidant capacity, and restored superoxide dismutases and glutathione peroxidase activities in H2O2-stimulated chondrocytes. In conclusion, our findings suggest that Hsd has the potential to improve antioxidant capacity in human chondrocytes.

AB - Excessive levels of oxidative stress can induce a senescence-associated secretory phenotype in chondrocytes which is characterized by the secretions of inflammatory mediators. Citrus flavonoid hesperidin (Hsd) is known to have anti-inflammatory and antioxidant capacities. Accordingly, we demonstrated the antioxidant property of Hsd against hydrogen peroxide (H2O2)-induced oxidative stress damages to chondrocytes in this study. Primary human chondrocytes were cultured in media supplemented with Hsd (5, 10, 50 and 100 μM) to evaluate the dose-dependent cytotoxicity. Subsequently, cells were stimulated by H2O2 and supplied with Hsd to study chondroprotective effects. Results showed that high-dose of Hsd (50 and 100 μM) has a deleterious effect on normal cells, whilst low Hsd (5 and 10 μM) supplements improved mitochondrial activity, cytotoxicity, proliferation kinetic, survival and cell senescence in H2O2-stimulated chondrocytes. Real-time PCR revealed that Hsd downregulated the mRNA levels of COX-2, IL-1β, TNF-α, MMP-3, MMP-9, and upregulated IL-10, TIMP-1, SOX9. Western blotting further revealed that Hsd modulates Foxo1, Foxo3 and Nrf2 signaling pathways. Furthermore, Hsd improved total antioxidant capacity, and restored superoxide dismutases and glutathione peroxidase activities in H2O2-stimulated chondrocytes. In conclusion, our findings suggest that Hsd has the potential to improve antioxidant capacity in human chondrocytes.

KW - Antioxidant

KW - Hesperidin

KW - Inflammation

KW - Osteoarthritis

KW - Oxidative stress

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