Effect of glutathione on DNA repair in cisplatin-resistant human ovarian cancer cell lines

Gi Ming Lai, Robert F. Ozols, Robert C. Young, Thomas C. Hamilton

研究成果: 雜誌貢獻文章同行評審

161 引文 斯高帕斯(Scopus)

摘要

We have studied the effect of glutathione reduction by buthionine sulf-oximine (BSO), a specific inhibitor ofλ -glutamyl cysteine synthetase, on DNA repair after cisplatin damage in an ovarian cancer cell line with invitro induced resistance to cisplatin. In addition, we have examined the effect of aphidicolin, a specific inhibitor of DNA polymerase a, in combination with BSO on cisplatin-associated DNA repair. BSO treatment was found topartially inhibit DNA repair, and the addition of aphidicolin caused nearly a100% inhibition in DNA repair activity. Treatment of cells with glutathioneester after BSO resulted in complete recovery of DNA repair activity or par-tial recovery if aphidicolin was present. The significance of these results to the chemosensitizing effects of BSO medi-ated glutathione reduction is discussed. [J Natl Cancer Inst 81:535-539, 1989].

原文英語
頁(從 - 到)535-539
頁數5
期刊Journal of the National Cancer Institute
81
發行號7
DOIs
出版狀態已發佈 - 四月 5 1989
對外發佈

ASJC Scopus subject areas

  • 腫瘤科
  • 癌症研究

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