摘要

Calcium sulfate has been used for bone regeneration in oral medicine. Osteopontin, a marker molecule of osteoblasts, plays multiple roles in bone formation. However, the effect of calcium sulfate on osteopontin expression in osteoblasts remains unclear. In this study, U-2 osteosarcoma (U-20S) osteoblast-like cells were cultured in medium containing 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate for 6 days. The messenger (m) RNA and protein expressions of osteopontin were respectively analyzed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting. In the in vitro culture for 1-2 days, there was no effect on the proliferation of cells, suggesting that calcium sulfate at up to 10μM is biocompatible. RT-PCR results showed that a lower concentration of calcium sulfate (< 1μM) upregulated the osteopontin mRNA level of U-2OS cells. However, the protein expression of osteopontin was downregulated as the concentration of calcium sulfate increased in cultured U-2OS osteoblastic cells. Dissolved •Ca(superscript 2+) and SO(superscript 2- subscript 4) from calcium sulfate hemihydrates may alter the structure of secreted osteopontin, which then was not recognizable by the antibody in the Western blot analysis. In summary, calcium sulfate can regulate the mRNA and protein expressions of osteopontin. Controlling the degradation of calcium sulfate implants is important for regulating bone formation. This study provides additional information to help dentists understand the effects of calcium sulfate on bone formation and use calcium sulfate more efficiently in treating bone defects.
原文英語
頁(從 - 到)30-37
頁數8
期刊Journal of Dental Sciences
3
發行號1
出版狀態已發佈 - 2008

指紋

Calcium Sulfate
Osteopontin
Osteosarcoma
Osteoblasts
Osteogenesis
Messenger RNA
Reverse Transcription
Western Blotting
Oral Medicine
Polymerase Chain Reaction
Proteins
Bone Regeneration
Dentists
Cultured Cells
Down-Regulation
Cell Proliferation

引用此文

Effect of Calcium Sulfate on Osteopontin Expression in U-2 Osteosarcoma Cells. / Chang, Yuan-Chieh; Wang, Duen-Jeng; Lee, Sheng-Yang; Chang, Wen-Chien; Yang, Wei-Chung Vivian.

於: Journal of Dental Sciences, 卷 3, 編號 1, 2008, p. 30-37.

研究成果: 雜誌貢獻文章

Chang, Yuan-Chieh ; Wang, Duen-Jeng ; Lee, Sheng-Yang ; Chang, Wen-Chien ; Yang, Wei-Chung Vivian. / Effect of Calcium Sulfate on Osteopontin Expression in U-2 Osteosarcoma Cells. 於: Journal of Dental Sciences. 2008 ; 卷 3, 編號 1. 頁 30-37.
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AU - Lee, Sheng-Yang

AU - Chang, Wen-Chien

AU - Yang, Wei-Chung Vivian

PY - 2008

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N2 - Calcium sulfate has been used for bone regeneration in oral medicine. Osteopontin, a marker molecule of osteoblasts, plays multiple roles in bone formation. However, the effect of calcium sulfate on osteopontin expression in osteoblasts remains unclear. In this study, U-2 osteosarcoma (U-20S) osteoblast-like cells were cultured in medium containing 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate for 6 days. The messenger (m) RNA and protein expressions of osteopontin were respectively analyzed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting. In the in vitro culture for 1-2 days, there was no effect on the proliferation of cells, suggesting that calcium sulfate at up to 10μM is biocompatible. RT-PCR results showed that a lower concentration of calcium sulfate (< 1μM) upregulated the osteopontin mRNA level of U-2OS cells. However, the protein expression of osteopontin was downregulated as the concentration of calcium sulfate increased in cultured U-2OS osteoblastic cells. Dissolved •Ca(superscript 2+) and SO(superscript 2- subscript 4) from calcium sulfate hemihydrates may alter the structure of secreted osteopontin, which then was not recognizable by the antibody in the Western blot analysis. In summary, calcium sulfate can regulate the mRNA and protein expressions of osteopontin. Controlling the degradation of calcium sulfate implants is important for regulating bone formation. This study provides additional information to help dentists understand the effects of calcium sulfate on bone formation and use calcium sulfate more efficiently in treating bone defects.

AB - Calcium sulfate has been used for bone regeneration in oral medicine. Osteopontin, a marker molecule of osteoblasts, plays multiple roles in bone formation. However, the effect of calcium sulfate on osteopontin expression in osteoblasts remains unclear. In this study, U-2 osteosarcoma (U-20S) osteoblast-like cells were cultured in medium containing 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate for 6 days. The messenger (m) RNA and protein expressions of osteopontin were respectively analyzed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting. In the in vitro culture for 1-2 days, there was no effect on the proliferation of cells, suggesting that calcium sulfate at up to 10μM is biocompatible. RT-PCR results showed that a lower concentration of calcium sulfate (< 1μM) upregulated the osteopontin mRNA level of U-2OS cells. However, the protein expression of osteopontin was downregulated as the concentration of calcium sulfate increased in cultured U-2OS osteoblastic cells. Dissolved •Ca(superscript 2+) and SO(superscript 2- subscript 4) from calcium sulfate hemihydrates may alter the structure of secreted osteopontin, which then was not recognizable by the antibody in the Western blot analysis. In summary, calcium sulfate can regulate the mRNA and protein expressions of osteopontin. Controlling the degradation of calcium sulfate implants is important for regulating bone formation. This study provides additional information to help dentists understand the effects of calcium sulfate on bone formation and use calcium sulfate more efficiently in treating bone defects.

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