Effect of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) hull extracts on testosterone release from rat leydig cells

Shih Min Hsia, Yi Wen Tseng, Shyi Wu Wang, Yueh Hsiung Kuo, Din Wen Huang, Paulus S. Wang, Wenchang Chiang

研究成果: 雜誌貢獻文章

13 引文 (Scopus)

摘要

Adlay has been used as a traditional Chinese medicine for the treatment of many diseases. However, few studies have reported the effects of adlay seeds on the endocrine system. In the present study, the effects of methanol extracts of adlay hull (AHM) on testosterone synthesis were studied. Rat Leydig cells were incubated with different reagents including human chorionic gonadotropin, 8-bromo-adenosine-3′,5′-cyclic monophosphate, forskolin, A23187, progesterone and androstenedione in the presence or absence of AHM. The rat anterior pituitary (AP) gland was treated with gonadotropin-releasing hormone (GnRH) in vitro in the presence or absence of AHM, and the concentrations of luteinizing hormone (LH) in the media were measured. AHM decreased testosterone release via the inhibition of (1) the PKA and PKC signal transduction pathways, (2) 17β-HSD enzyme activity in rat Leydig cells, and (3) in vitro GnRH-induced LH secretion.

原文英語
頁(從 - 到)687-695
頁數9
期刊Phytotherapy Research
23
發行號5
DOIs
出版狀態已發佈 - 五月 2009
對外發佈Yes

指紋

Coix
Leydig Cells
Testosterone
Luteinizing Hormone
Gonadotropin-Releasing Hormone
Endocrine System
Anterior Pituitary Gland
Androstenedione
Chinese Traditional Medicine
Calcimycin
Colforsin
Chorionic Gonadotropin
Adenosine
Progesterone
Methanol
Signal Transduction
Seeds
Enzymes
In Vitro Techniques
Therapeutics

ASJC Scopus subject areas

  • Pharmacology
  • Medicine(all)

引用此文

Effect of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) hull extracts on testosterone release from rat leydig cells. / Hsia, Shih Min; Tseng, Yi Wen; Wang, Shyi Wu; Kuo, Yueh Hsiung; Huang, Din Wen; Wang, Paulus S.; Chiang, Wenchang.

於: Phytotherapy Research, 卷 23, 編號 5, 05.2009, p. 687-695.

研究成果: 雜誌貢獻文章

Hsia, Shih Min ; Tseng, Yi Wen ; Wang, Shyi Wu ; Kuo, Yueh Hsiung ; Huang, Din Wen ; Wang, Paulus S. ; Chiang, Wenchang. / Effect of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) hull extracts on testosterone release from rat leydig cells. 於: Phytotherapy Research. 2009 ; 卷 23, 編號 5. 頁 687-695.
@article{01968ac98c404378aea318c188cb722e,
title = "Effect of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) hull extracts on testosterone release from rat leydig cells",
abstract = "Adlay has been used as a traditional Chinese medicine for the treatment of many diseases. However, few studies have reported the effects of adlay seeds on the endocrine system. In the present study, the effects of methanol extracts of adlay hull (AHM) on testosterone synthesis were studied. Rat Leydig cells were incubated with different reagents including human chorionic gonadotropin, 8-bromo-adenosine-3′,5′-cyclic monophosphate, forskolin, A23187, progesterone and androstenedione in the presence or absence of AHM. The rat anterior pituitary (AP) gland was treated with gonadotropin-releasing hormone (GnRH) in vitro in the presence or absence of AHM, and the concentrations of luteinizing hormone (LH) in the media were measured. AHM decreased testosterone release via the inhibition of (1) the PKA and PKC signal transduction pathways, (2) 17β-HSD enzyme activity in rat Leydig cells, and (3) in vitro GnRH-induced LH secretion.",
keywords = "Adlay hulls, Leydig cell, Testosterone",
author = "Hsia, {Shih Min} and Tseng, {Yi Wen} and Wang, {Shyi Wu} and Kuo, {Yueh Hsiung} and Huang, {Din Wen} and Wang, {Paulus S.} and Wenchang Chiang",
year = "2009",
month = "5",
doi = "10.1002/ptr.2706",
language = "English",
volume = "23",
pages = "687--695",
journal = "Phytotherapy Research",
issn = "0951-418X",
publisher = "John Wiley and Sons Ltd",
number = "5",

}

TY - JOUR

T1 - Effect of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) hull extracts on testosterone release from rat leydig cells

AU - Hsia, Shih Min

AU - Tseng, Yi Wen

AU - Wang, Shyi Wu

AU - Kuo, Yueh Hsiung

AU - Huang, Din Wen

AU - Wang, Paulus S.

AU - Chiang, Wenchang

PY - 2009/5

Y1 - 2009/5

N2 - Adlay has been used as a traditional Chinese medicine for the treatment of many diseases. However, few studies have reported the effects of adlay seeds on the endocrine system. In the present study, the effects of methanol extracts of adlay hull (AHM) on testosterone synthesis were studied. Rat Leydig cells were incubated with different reagents including human chorionic gonadotropin, 8-bromo-adenosine-3′,5′-cyclic monophosphate, forskolin, A23187, progesterone and androstenedione in the presence or absence of AHM. The rat anterior pituitary (AP) gland was treated with gonadotropin-releasing hormone (GnRH) in vitro in the presence or absence of AHM, and the concentrations of luteinizing hormone (LH) in the media were measured. AHM decreased testosterone release via the inhibition of (1) the PKA and PKC signal transduction pathways, (2) 17β-HSD enzyme activity in rat Leydig cells, and (3) in vitro GnRH-induced LH secretion.

AB - Adlay has been used as a traditional Chinese medicine for the treatment of many diseases. However, few studies have reported the effects of adlay seeds on the endocrine system. In the present study, the effects of methanol extracts of adlay hull (AHM) on testosterone synthesis were studied. Rat Leydig cells were incubated with different reagents including human chorionic gonadotropin, 8-bromo-adenosine-3′,5′-cyclic monophosphate, forskolin, A23187, progesterone and androstenedione in the presence or absence of AHM. The rat anterior pituitary (AP) gland was treated with gonadotropin-releasing hormone (GnRH) in vitro in the presence or absence of AHM, and the concentrations of luteinizing hormone (LH) in the media were measured. AHM decreased testosterone release via the inhibition of (1) the PKA and PKC signal transduction pathways, (2) 17β-HSD enzyme activity in rat Leydig cells, and (3) in vitro GnRH-induced LH secretion.

KW - Adlay hulls

KW - Leydig cell

KW - Testosterone

UR - http://www.scopus.com/inward/record.url?scp=65649124104&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65649124104&partnerID=8YFLogxK

U2 - 10.1002/ptr.2706

DO - 10.1002/ptr.2706

M3 - Article

C2 - 19107733

AN - SCOPUS:65649124104

VL - 23

SP - 687

EP - 695

JO - Phytotherapy Research

JF - Phytotherapy Research

SN - 0951-418X

IS - 5

ER -