Effect of Acetylsalicylic Acid on Oxidative Stress-Related Damage of INS-1 Pancreatic Beta Cells

Yan-Yu Lin, Yu-Shan Hsieh

研究成果: 雜誌貢獻文章同行評審

摘要

Background: Diabetes is becoming a serious threat to human health worldwide and has affected quality of life in Taiwan. Acetylsalicylic acid (ASA) is a commonly used drug for preventing cardiovascular disease in patients with type 2 diabetes mellitus in Taiwan. However, the effect of ASA on the reduction of the defection of beta cells remains unclear. Method: The protective effects of ASA on the INS-1 cell line was evaluated following the exposure of the cells to normal glucose level (11 mM) or high glucose level (33 mM) to mimic normoglycemia and hyperglycemia situations, respectively. Possible action mechanisms were determined by analyzing the generation of reactive oxygen species (ROS), nitrites, and lactate dehydrogenase (LDH). Results: Although the effect of ASA was not significant in the normal glucose condition, ASA significantly reversed the elevation of ROS, nitrites production, and LDH levels induced by high glucose in INS-1 cells. Conclusions: ASA results in anti-cell damage under hyperglycemic conditions through a decrease in LDH levels. However, additional studies using diabetes models are required to validate this finding and investigate the underlying mechanism. Furthermore, the manipulation of intracellular LDH levels in β-cells are a potential therapeutic focus for hyperglycemia. Moreover, LDH level is a potential marker of β-cell damage.
原文英語
頁(從 - 到)94-99
頁數6
期刊Formosan Journal of Endocrinology and Metabolism
12
發行號2
DOIs
出版狀態已發佈 - 12月 2021

指紋

深入研究「Effect of Acetylsalicylic Acid on Oxidative Stress-Related Damage of INS-1 Pancreatic Beta Cells」主題。共同形成了獨特的指紋。

引用此