EBV DNA polymerase inhibition of tannins from Eugenia uniflora

Mei Hsien Lee, Jwo Farn Chiou, Kun Ying Yen, Ling-Ling Yang

研究成果: 雜誌貢獻文章同行評審

45 引文 斯高帕斯(Scopus)

摘要

Nasopharyngeal carcinoma (NPC) is one of the high population malignant tumors among Chinese in southern China and southeast Asia. Epstein-Barr virus (EBV) is a human B lymphotropic herpes virus which is known to be closely associated with NPC. EBV DNA polymerase is a key enzyme during EBV replication and is measured by its radioactivity. The addition of phorbol 12-myristate 13-acetate to Raji cell cultures led to a large increase in EBV DNA polymerase, which was purified by sequential DEAE-cellulose, phosphocellulose and DNA-cellulose column chromatography. Four tannins were isolated from the active fractions of Eugenia uniflora L., which were tested for the inhibition of EBV DNA polymerase. The results showed the 50% inhibitory concentration (IC50) values of gallocatechin, oenothein B, eugeniflorins D1 and D2 were 26.5 62.3, 3.0 and 3.5 μM, respectively. Furthermore, when compared with the positive control (phosphonoacetic acid), an inhibitor of EBV replication, the IC50 value was 16.4 μM. In view of the results, eugeniflorins D1 and D2 are the potency principles in the inhibition of EBV DNA polymerase from E. uniflora. Copyright (C) 2000 Published by Elsevier Science Ireland Ltd.

原文英語
頁(從 - 到)131-136
頁數6
期刊Cancer Letters
154
發行號2
DOIs
出版狀態已發佈 - 6月 30 2000

ASJC Scopus subject areas

  • 癌症研究
  • 分子生物學
  • 腫瘤科

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