E2F6 functions as a competing endogenous RNA, and transcriptional repressor, to promote ovarian cancer stemness

Frank H.C. Cheng, Hon Yi Lin, Tzy Wei Hwang, Yin Chen Chen, Rui Lan Huang, Chia Bin Chang, Weiqin Yang, Ru Inn Lin, Ching Wen Lin, Gary C.W. Chen, Shu Yuan Mai, Jora M.J. Lin, Yu Ming Chuang, Jian Liang Chou, Li Wei Kuo, Chin Li, Alfred S.L. Cheng, Hung Cheng Lai, Shu Fen Wu, Je Chiang Tsai & 1 others Michael W.Y. Chan

研究成果: 雜誌貢獻文章

2 引文 (Scopus)

摘要

Ovarian cancer is the most lethal cancer of the female reproductive system. In that regard, several epidemiological studies suggest that long-term exposure to estrogen could increase ovarian cancer risk, although its precise role remains controversial. To decipher a mechanism for this, we previously generated a mathematical model of how estrogen-mediated upregulation of the transcription factor, E2F6, upregulates the ovarian cancer stem/initiating cell marker, c-Kit, by epigenetic silencing the tumor suppressor miR-193a, and a competing endogenous (ceRNA) mechanism. In this study, we tested that previous mathematical model, showing that estrogen treatment of immortalized ovarian surface epithelial cells upregulated both E2F6 and c-KIT, but downregulated miR-193a. Luciferase assays further confirmed that microRNA-193a targets both E2F6 and c-Kit. Interestingly, ChIP-PCR and bisulphite pyrosequencing showed that E2F6 also epigenetically suppresses miR-193a, through recruitment of EZH2, and by a complex ceRNA mechanism in ovarian cancer cell lines. Importantly, cell line and animal experiments both confirmed that E2F6 promotes ovarian cancer stemness, whereas E2F6 or EZH2 depletion derepressed miR-193a, which opposes cancer stemness, by alleviating DNA methylation and repressive chromatin. Finally, 118 ovarian cancer patients with miR-193a promoter hypermethylation had poorer survival than those without hypermethylation. These results suggest that an estrogen-mediated E2F6 ceRNA network epigenetically and competitively inhibits microRNA-193a activity, promoting ovarian cancer stemness and tumorigenesis.
原文英語
期刊Cancer Science
DOIs
出版狀態接受/付印 - 一月 1 2019

指紋

Ovarian Neoplasms
RNA
Estrogens
E2F6 Transcription Factor
MicroRNAs
Theoretical Models
Up-Regulation
Cell Line
Neoplasms
Neoplastic Stem Cells
DNA Methylation
Luciferases
Epigenomics
Chromatin
Epidemiologic Studies
Carcinogenesis
Down-Regulation
Epithelial Cells
Polymerase Chain Reaction
Survival

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

引用此文

Cheng, F. H. C., Lin, H. Y., Hwang, T. W., Chen, Y. C., Huang, R. L., Chang, C. B., ... Chan, M. W. Y. (認可的出版社/出版中). E2F6 functions as a competing endogenous RNA, and transcriptional repressor, to promote ovarian cancer stemness. Cancer Science. https://doi.org/10.1111/cas.13920

E2F6 functions as a competing endogenous RNA, and transcriptional repressor, to promote ovarian cancer stemness. / Cheng, Frank H.C.; Lin, Hon Yi; Hwang, Tzy Wei; Chen, Yin Chen; Huang, Rui Lan; Chang, Chia Bin; Yang, Weiqin; Lin, Ru Inn; Lin, Ching Wen; Chen, Gary C.W.; Mai, Shu Yuan; Lin, Jora M.J.; Chuang, Yu Ming; Chou, Jian Liang; Kuo, Li Wei; Li, Chin; Cheng, Alfred S.L.; Lai, Hung Cheng; Wu, Shu Fen; Tsai, Je Chiang; Chan, Michael W.Y.

於: Cancer Science, 01.01.2019.

研究成果: 雜誌貢獻文章

Cheng, FHC, Lin, HY, Hwang, TW, Chen, YC, Huang, RL, Chang, CB, Yang, W, Lin, RI, Lin, CW, Chen, GCW, Mai, SY, Lin, JMJ, Chuang, YM, Chou, JL, Kuo, LW, Li, C, Cheng, ASL, Lai, HC, Wu, SF, Tsai, JC & Chan, MWY 2019, 'E2F6 functions as a competing endogenous RNA, and transcriptional repressor, to promote ovarian cancer stemness', Cancer Science. https://doi.org/10.1111/cas.13920
Cheng, Frank H.C. ; Lin, Hon Yi ; Hwang, Tzy Wei ; Chen, Yin Chen ; Huang, Rui Lan ; Chang, Chia Bin ; Yang, Weiqin ; Lin, Ru Inn ; Lin, Ching Wen ; Chen, Gary C.W. ; Mai, Shu Yuan ; Lin, Jora M.J. ; Chuang, Yu Ming ; Chou, Jian Liang ; Kuo, Li Wei ; Li, Chin ; Cheng, Alfred S.L. ; Lai, Hung Cheng ; Wu, Shu Fen ; Tsai, Je Chiang ; Chan, Michael W.Y. / E2F6 functions as a competing endogenous RNA, and transcriptional repressor, to promote ovarian cancer stemness. 於: Cancer Science. 2019.
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abstract = "Ovarian cancer is the most lethal cancer of the female reproductive system. In that regard, several epidemiological studies suggest that long-term exposure to estrogen could increase ovarian cancer risk, although its precise role remains controversial. To decipher a mechanism for this, we previously generated a mathematical model of how estrogen-mediated upregulation of the transcription factor, E2F6, upregulates the ovarian cancer stem/initiating cell marker, c-Kit, by epigenetic silencing the tumor suppressor miR-193a, and a competing endogenous (ceRNA) mechanism. In this study, we tested that previous mathematical model, showing that estrogen treatment of immortalized ovarian surface epithelial cells upregulated both E2F6 and c-KIT, but downregulated miR-193a. Luciferase assays further confirmed that microRNA-193a targets both E2F6 and c-Kit. Interestingly, ChIP-PCR and bisulphite pyrosequencing showed that E2F6 also epigenetically suppresses miR-193a, through recruitment of EZH2, and by a complex ceRNA mechanism in ovarian cancer cell lines. Importantly, cell line and animal experiments both confirmed that E2F6 promotes ovarian cancer stemness, whereas E2F6 or EZH2 depletion derepressed miR-193a, which opposes cancer stemness, by alleviating DNA methylation and repressive chromatin. Finally, 118 ovarian cancer patients with miR-193a promoter hypermethylation had poorer survival than those without hypermethylation. These results suggest that an estrogen-mediated E2F6 ceRNA network epigenetically and competitively inhibits microRNA-193a activity, promoting ovarian cancer stemness and tumorigenesis.",
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T1 - E2F6 functions as a competing endogenous RNA, and transcriptional repressor, to promote ovarian cancer stemness

AU - Cheng, Frank H.C.

AU - Lin, Hon Yi

AU - Hwang, Tzy Wei

AU - Chen, Yin Chen

AU - Huang, Rui Lan

AU - Chang, Chia Bin

AU - Yang, Weiqin

AU - Lin, Ru Inn

AU - Lin, Ching Wen

AU - Chen, Gary C.W.

AU - Mai, Shu Yuan

AU - Lin, Jora M.J.

AU - Chuang, Yu Ming

AU - Chou, Jian Liang

AU - Kuo, Li Wei

AU - Li, Chin

AU - Cheng, Alfred S.L.

AU - Lai, Hung Cheng

AU - Wu, Shu Fen

AU - Tsai, Je Chiang

AU - Chan, Michael W.Y.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Ovarian cancer is the most lethal cancer of the female reproductive system. In that regard, several epidemiological studies suggest that long-term exposure to estrogen could increase ovarian cancer risk, although its precise role remains controversial. To decipher a mechanism for this, we previously generated a mathematical model of how estrogen-mediated upregulation of the transcription factor, E2F6, upregulates the ovarian cancer stem/initiating cell marker, c-Kit, by epigenetic silencing the tumor suppressor miR-193a, and a competing endogenous (ceRNA) mechanism. In this study, we tested that previous mathematical model, showing that estrogen treatment of immortalized ovarian surface epithelial cells upregulated both E2F6 and c-KIT, but downregulated miR-193a. Luciferase assays further confirmed that microRNA-193a targets both E2F6 and c-Kit. Interestingly, ChIP-PCR and bisulphite pyrosequencing showed that E2F6 also epigenetically suppresses miR-193a, through recruitment of EZH2, and by a complex ceRNA mechanism in ovarian cancer cell lines. Importantly, cell line and animal experiments both confirmed that E2F6 promotes ovarian cancer stemness, whereas E2F6 or EZH2 depletion derepressed miR-193a, which opposes cancer stemness, by alleviating DNA methylation and repressive chromatin. Finally, 118 ovarian cancer patients with miR-193a promoter hypermethylation had poorer survival than those without hypermethylation. These results suggest that an estrogen-mediated E2F6 ceRNA network epigenetically and competitively inhibits microRNA-193a activity, promoting ovarian cancer stemness and tumorigenesis.

AB - Ovarian cancer is the most lethal cancer of the female reproductive system. In that regard, several epidemiological studies suggest that long-term exposure to estrogen could increase ovarian cancer risk, although its precise role remains controversial. To decipher a mechanism for this, we previously generated a mathematical model of how estrogen-mediated upregulation of the transcription factor, E2F6, upregulates the ovarian cancer stem/initiating cell marker, c-Kit, by epigenetic silencing the tumor suppressor miR-193a, and a competing endogenous (ceRNA) mechanism. In this study, we tested that previous mathematical model, showing that estrogen treatment of immortalized ovarian surface epithelial cells upregulated both E2F6 and c-KIT, but downregulated miR-193a. Luciferase assays further confirmed that microRNA-193a targets both E2F6 and c-Kit. Interestingly, ChIP-PCR and bisulphite pyrosequencing showed that E2F6 also epigenetically suppresses miR-193a, through recruitment of EZH2, and by a complex ceRNA mechanism in ovarian cancer cell lines. Importantly, cell line and animal experiments both confirmed that E2F6 promotes ovarian cancer stemness, whereas E2F6 or EZH2 depletion derepressed miR-193a, which opposes cancer stemness, by alleviating DNA methylation and repressive chromatin. Finally, 118 ovarian cancer patients with miR-193a promoter hypermethylation had poorer survival than those without hypermethylation. These results suggest that an estrogen-mediated E2F6 ceRNA network epigenetically and competitively inhibits microRNA-193a activity, promoting ovarian cancer stemness and tumorigenesis.

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KW - ovarian cancer

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