Dual roles of 17-β estradiol in estrogen receptor-dependent growth inhibition in renal cell carcinoma

Kuo Chiang Chen, Chih Ming Lin, Chi Jung Huang, Shao Kuan Chen, Sheng Tang Wu, Han-Sun Chiang, Wei Chi Ku

研究成果: 雜誌貢獻文章

11 引文 斯高帕斯(Scopus)

摘要

Background: It has been proposed that 17-β- estradiol (E2) activates estrogen receptor and inhibits renal cell carcinoma (RCC) growth. In the present study we explored the role of E2 and ER in the regulation of RCC growth. Materials and Methods: The RCC cell line ACHN was treated by E2 combining with E2 antagonist Fulvestrant or ER knockdown, and cell growth was monitored. Quantitative phosphoproteomics was applied to study the E2 regulated non-genomic phosphorylation changes. Western blotting, immunofluorescence microscopy, and apoptosis assays were used for validation. Results: E2 induced ERdependent growth inhibition in RCC cell lines. Quantitative phosphoproteomics revealed that E2 induced both apoptosis and autophagy. Cellular apoptosis was confirmed by altered.
原文英語
頁(從 - 到)219-230
頁數12
期刊Cancer Genomics and Proteomics
13
發行號3
出版狀態已發佈 - 五月 1 2016

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Cancer Research
  • Biochemistry

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    Chen, K. C., Lin, C. M., Huang, C. J., Chen, S. K., Wu, S. T., Chiang, H-S., & Ku, W. C. (2016). Dual roles of 17-β estradiol in estrogen receptor-dependent growth inhibition in renal cell carcinoma. Cancer Genomics and Proteomics, 13(3), 219-230.