Dual-compartmental transcriptomic + proteomic analysis of a marine endosymbiosis exposed to environmental change

Anderson B. Mayfield, Yu Bin Wang, Chii Shiarng Chen, Shu Hwa Chen, Chung Yen Lin

研究成果: 雜誌貢獻文章

17 引文 斯高帕斯(Scopus)

摘要

As significant anthropogenic pressures are putting undue stress on the world's oceans, there has been a concerted effort to understand how marine organisms respond to environmental change. Transcriptomic approaches, in particular, have been readily employed to document the mRNA-level response of a plethora of marine invertebrates exposed to an array of simulated stress scenarios, with the tacit and untested assumption being that the respective proteins show a corresponding trend. To better understand the degree of congruency between mRNA and protein expression in an endosymbiotic marine invertebrate, mRNAs and proteins were sequenced from the same samples of the common, Indo-Pacific coral Seriatopora hystrix exposed to stable or upwelling-simulating conditions for 1 week. Of the 167 proteins downregulated at variable temperature, only two were associated with mRNAs that were also differentially expressed between treatments. Of the 378 differentially expressed genes, none were associated with a differentially expressed protein. Collectively, these results highlight the inherent risk of inferring cellular behaviour based on mRNA expression data alone and challenge the current, mRNA-focused approach taken by most marine and many molecular biologists.
原文英語
頁(從 - 到)5944-5958
頁數15
期刊Molecular Ecology
25
發行號23
DOIs
出版狀態已發佈 - 十二月 1 2016
對外發佈Yes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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