Down-regulation of Endogenous Antioxidant Enzymes Gene Expression by Supplement of Vitamin C in Cultured Pheochromocytoma Cells

Hung Y. Yang, Jun Jack Cheng, Tzu-Hurng Cheng, Ju C. Liu, Paul Chan, Juei Tang Cheng

研究成果: 雜誌貢獻文章

摘要

Background: Reactive oxygen species (ROS) have been linked to a variety of pathophysiological changes. Epidemiological studies supported the beneficial effect of supplementation of antioxidants. Antioxidant enzymes including catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPX) are endogenous enzymes which can scavenge ROS. This study investigated the effect of supplementation of ascorbic acid (vitamin C) on the changes of catalase, SOD, and GPX gene expression in cultured pheochromocytoma cells. Methods: Pheochromocytoma cells were incubated with vitamin C and divided into 4 groups: a control group (without vitamin C) and 3 treatment groups with vitamin C at 40, 80 and 160 μmol/L, respectively. After short-term (2 days) and long-term (7 days) incubation, the catalase, SOD, and GPX mRNA levels were determined by Northern blot. Results: After 2 days of incubation, vitamin C (160 μmol/L) attenuated the GPX mRNA level (100% to 91.8 ± 3.8%, p <0.01), whereas the catalase and SOD mRNA remained unchanged. After 7 days' incubation with vitamin C, all the catalase, SOD, and GPX mRNA were decreased significantly. Conclusion: These results suggest that 2 and 7 days of incubation with relatively high concentrations of vitamin C may down-regulate gene expression of antioxidant enzymes in cultured pheochromocytoma cells. Whether this phenomenon implicates clinical benefit needs further evaluation.
原文英語
頁(從 - 到)143-148
頁數6
期刊Acta Cardiologica Sinica
19
發行號3
出版狀態已發佈 - 九月 2003

指紋

Pheochromocytoma
Ascorbic Acid
Cultured Cells
Down-Regulation
Antioxidants
Gene Expression
Glutathione Peroxidase
Catalase
Superoxide Dismutase
Enzymes
Messenger RNA
Reactive Oxygen Species
Northern Blotting
Epidemiologic Studies
Control Groups

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

引用此文

Down-regulation of Endogenous Antioxidant Enzymes Gene Expression by Supplement of Vitamin C in Cultured Pheochromocytoma Cells. / Yang, Hung Y.; Cheng, Jun Jack; Cheng, Tzu-Hurng; Liu, Ju C.; Chan, Paul; Cheng, Juei Tang.

於: Acta Cardiologica Sinica, 卷 19, 編號 3, 09.2003, p. 143-148.

研究成果: 雜誌貢獻文章

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abstract = "Background: Reactive oxygen species (ROS) have been linked to a variety of pathophysiological changes. Epidemiological studies supported the beneficial effect of supplementation of antioxidants. Antioxidant enzymes including catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPX) are endogenous enzymes which can scavenge ROS. This study investigated the effect of supplementation of ascorbic acid (vitamin C) on the changes of catalase, SOD, and GPX gene expression in cultured pheochromocytoma cells. Methods: Pheochromocytoma cells were incubated with vitamin C and divided into 4 groups: a control group (without vitamin C) and 3 treatment groups with vitamin C at 40, 80 and 160 μmol/L, respectively. After short-term (2 days) and long-term (7 days) incubation, the catalase, SOD, and GPX mRNA levels were determined by Northern blot. Results: After 2 days of incubation, vitamin C (160 μmol/L) attenuated the GPX mRNA level (100{\%} to 91.8 ± 3.8{\%}, p <0.01), whereas the catalase and SOD mRNA remained unchanged. After 7 days' incubation with vitamin C, all the catalase, SOD, and GPX mRNA were decreased significantly. Conclusion: These results suggest that 2 and 7 days of incubation with relatively high concentrations of vitamin C may down-regulate gene expression of antioxidant enzymes in cultured pheochromocytoma cells. Whether this phenomenon implicates clinical benefit needs further evaluation.",
keywords = "Catalase, Glutathione peroxidase, Pheochromocytoma cells, Reactive oxygen species, Superoxide dismutase, Vitamin C",
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T1 - Down-regulation of Endogenous Antioxidant Enzymes Gene Expression by Supplement of Vitamin C in Cultured Pheochromocytoma Cells

AU - Yang, Hung Y.

AU - Cheng, Jun Jack

AU - Cheng, Tzu-Hurng

AU - Liu, Ju C.

AU - Chan, Paul

AU - Cheng, Juei Tang

PY - 2003/9

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N2 - Background: Reactive oxygen species (ROS) have been linked to a variety of pathophysiological changes. Epidemiological studies supported the beneficial effect of supplementation of antioxidants. Antioxidant enzymes including catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPX) are endogenous enzymes which can scavenge ROS. This study investigated the effect of supplementation of ascorbic acid (vitamin C) on the changes of catalase, SOD, and GPX gene expression in cultured pheochromocytoma cells. Methods: Pheochromocytoma cells were incubated with vitamin C and divided into 4 groups: a control group (without vitamin C) and 3 treatment groups with vitamin C at 40, 80 and 160 μmol/L, respectively. After short-term (2 days) and long-term (7 days) incubation, the catalase, SOD, and GPX mRNA levels were determined by Northern blot. Results: After 2 days of incubation, vitamin C (160 μmol/L) attenuated the GPX mRNA level (100% to 91.8 ± 3.8%, p <0.01), whereas the catalase and SOD mRNA remained unchanged. After 7 days' incubation with vitamin C, all the catalase, SOD, and GPX mRNA were decreased significantly. Conclusion: These results suggest that 2 and 7 days of incubation with relatively high concentrations of vitamin C may down-regulate gene expression of antioxidant enzymes in cultured pheochromocytoma cells. Whether this phenomenon implicates clinical benefit needs further evaluation.

AB - Background: Reactive oxygen species (ROS) have been linked to a variety of pathophysiological changes. Epidemiological studies supported the beneficial effect of supplementation of antioxidants. Antioxidant enzymes including catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPX) are endogenous enzymes which can scavenge ROS. This study investigated the effect of supplementation of ascorbic acid (vitamin C) on the changes of catalase, SOD, and GPX gene expression in cultured pheochromocytoma cells. Methods: Pheochromocytoma cells were incubated with vitamin C and divided into 4 groups: a control group (without vitamin C) and 3 treatment groups with vitamin C at 40, 80 and 160 μmol/L, respectively. After short-term (2 days) and long-term (7 days) incubation, the catalase, SOD, and GPX mRNA levels were determined by Northern blot. Results: After 2 days of incubation, vitamin C (160 μmol/L) attenuated the GPX mRNA level (100% to 91.8 ± 3.8%, p <0.01), whereas the catalase and SOD mRNA remained unchanged. After 7 days' incubation with vitamin C, all the catalase, SOD, and GPX mRNA were decreased significantly. Conclusion: These results suggest that 2 and 7 days of incubation with relatively high concentrations of vitamin C may down-regulate gene expression of antioxidant enzymes in cultured pheochromocytoma cells. Whether this phenomenon implicates clinical benefit needs further evaluation.

KW - Catalase

KW - Glutathione peroxidase

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