摘要
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 194-201 |
| 頁數 | 8 |
| 期刊 | Food and Function |
| 卷 | 7 |
| 發行號 | 1 |
| DOIs | |
| 出版狀態 | 已發佈 - 一月 1 2016 |
指紋
ASJC Scopus subject areas
- Food Science
引用此文
Dose-related effects of ferric citrate supplementation on endoplasmic reticular stress responses and insulin signalling pathways in streptozotocin-nicotinamide-induced diabetes. / Liu, Kai Li; Chen, Pei Yin; Wang, Chi Mei; Chen, Wei-Yu; Chen, Chia Wen; Owaga, E. E.; Chang, Jung-Su.
於: Food and Function, 卷 7, 編號 1, 01.01.2016, p. 194-201.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Dose-related effects of ferric citrate supplementation on endoplasmic reticular stress responses and insulin signalling pathways in streptozotocin-nicotinamide-induced diabetes
AU - Liu, Kai Li
AU - Chen, Pei Yin
AU - Wang, Chi Mei
AU - Chen, Wei-Yu
AU - Chen, Chia Wen
AU - Owaga, E. E.
AU - Chang, Jung-Su
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Diabetic patients are at high risk of developing anemia; however, pharmacological doses of iron supplementation may vary greatly depending on diabetes-related complications. The aim of this study was to investigate the dose-dependent effect of iron on glucose disposal with a special focus on endoplasmic reticular (ER) stress, iron metabolism, and insulin signalling pathways. Diabetes was induced in overnight fasted rats by intraperitoneal (i.p.) injections of 40 mg kg-1 streptozotocin (STZ) and 100 mg kg-1 nicotinamide. Diabetic rats were fed a standard diet (36.7 mg ferric iron per kg diet) or pharmacological doses of ferric citrate (0.5, 1, 2, and 3 g ferric iron per kg diet). Ferric citrate supplementation showed a dose-related effect on hepatic ER stress responses and total iron levels, which were associated with increased hepcidin and decreased ferroportin expressions. Iron-fed rats had increased sizes of their pancreatic islets and hyperinsulinemia compared to rats fed a standard diet. A western blot analysis revealed that iron feeding decreased total insulin receptor substrate 1 (IRS1), phosphorylated IRS1ser307, and AS160 but increased phosphorylated GSK-3β. Iron supplementation inhibited the nuclear translocation of AKT but promoted FOXO1 translocation to nuclei. Ferric citrate supplementation showed a dose-related effect on ER stress responses, hepatic iron, and the insulin signaling pathway. Adverse effects were more evident at high iron doses (>1 g ferric iron per kg diet), which is equivalent to a 60 kg human male consuming >500 mg elemental iron per day.
AB - Diabetic patients are at high risk of developing anemia; however, pharmacological doses of iron supplementation may vary greatly depending on diabetes-related complications. The aim of this study was to investigate the dose-dependent effect of iron on glucose disposal with a special focus on endoplasmic reticular (ER) stress, iron metabolism, and insulin signalling pathways. Diabetes was induced in overnight fasted rats by intraperitoneal (i.p.) injections of 40 mg kg-1 streptozotocin (STZ) and 100 mg kg-1 nicotinamide. Diabetic rats were fed a standard diet (36.7 mg ferric iron per kg diet) or pharmacological doses of ferric citrate (0.5, 1, 2, and 3 g ferric iron per kg diet). Ferric citrate supplementation showed a dose-related effect on hepatic ER stress responses and total iron levels, which were associated with increased hepcidin and decreased ferroportin expressions. Iron-fed rats had increased sizes of their pancreatic islets and hyperinsulinemia compared to rats fed a standard diet. A western blot analysis revealed that iron feeding decreased total insulin receptor substrate 1 (IRS1), phosphorylated IRS1ser307, and AS160 but increased phosphorylated GSK-3β. Iron supplementation inhibited the nuclear translocation of AKT but promoted FOXO1 translocation to nuclei. Ferric citrate supplementation showed a dose-related effect on ER stress responses, hepatic iron, and the insulin signaling pathway. Adverse effects were more evident at high iron doses (>1 g ferric iron per kg diet), which is equivalent to a 60 kg human male consuming >500 mg elemental iron per day.
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U2 - 10.1039/c5fo01252j
DO - 10.1039/c5fo01252j
M3 - Article
C2 - 26611621
AN - SCOPUS:84956633163
VL - 7
SP - 194
EP - 201
JO - Food and Function
JF - Food and Function
SN - 2042-6496
IS - 1
ER -