DNA rearrangement mediated by inverted repeats

研究成果: 雜誌貢獻文章

76 引文 斯高帕斯(Scopus)


Inverted repeats of DNA are widespread in the genomes of eukaryotes and prokaryotes and can mediate genome rearrangement. We studied rearrangement mediated by plasmid-borne inverted repeats in Escherichia coli. We show that inverted repeats can mediate an efficient and recA-independent recombination event. Surprisingly, the product of this recombination is not that of simple inversion between the inverted repeats, but almost exclusively an unusual head-to-head dimer with complex DNA rearrangement. Moreover, this recombination is dramatically reduced by increasing the distance separating the repeats. These results can be readily explained by a model involving reciprocal switching of the leading and lagging strands of DNA replication within the inverted repeats, which leads to the formation of a Holliday junction. Reciprocal strand switching during DNA replication might be a common mechanism for genome rearrangement associated with inverted duplication.

頁(從 - 到)819-823
期刊Proceedings of the National Academy of Sciences of the United States of America
出版狀態已發佈 - 一月 23 1996

ASJC Scopus subject areas

  • General
  • Genetics

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