Divaricoside Exerts Antitumor Effects, in Part, by Modulating Mcl-1 in Human Oral Squamous Cell Carcinoma Cells

Jing Ru Weng, Li Yuan Bai, Shih Jiuan Chiu, Chang Fang Chiu, Wei Yu Lin, Jing Lan Hu, Tzong Ming Shieh

研究成果: 雜誌貢獻文章

摘要

Cardiac glycosides (CGs), prescribed to treat congestive heart failure and arrhythmias, exert potent antitumor activity. In this study, divaricoside (DIV), a CG isolated from Strophanthus divaricatus was examined for its antitumor potency in oral squamous cell carcinoma (OSCC) cells. Cell growth was inhibited by DIV in a dose- and time-dependent manner in SCC2095 and OECM-1 OSCC cells using MTT assays. DIV induced S and G2/M phase arrest accompanied by downregulation of phosphorylated CDC25C, CDC25C, and CDC2 in SCC2095 cells. In addition, DIV induced apoptosis by activating caspase-3 and downregulating the expression of Mcl-1. Furthermore, overexpression of Mcl-1 partially reversed DIV-induced death in SCC2095 cells. Additionally, western blot and transmission electron microscopy analyses also indicated that DIV induced autophagy in SCC2095 cells. However, the combination of autophagy inhibitor did not affect DIV-mediated apoptosis in SCC2095 cells. Together, these findings suggest that translational potential of DIV to be developed as a therapeutic agent for OSCC treatment.
原文英語
頁(從 - 到)151-159
頁數9
期刊Computational and Structural Biotechnology Journal
17
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

Glycosides
Squamous Cell Carcinoma
Cardiac Glycosides
Cells
Cell death
Apoptosis
Cell growth
Caspase 3
Autophagy
Assays
Strophanthus
Transmission electron microscopy
Down-Regulation
G2 Phase
Epithelial Cells
Transmission Electron Microscopy
Cell Division
Cardiac Arrhythmias
Heart Failure
Western Blotting

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Computer Science Applications

引用此文

Divaricoside Exerts Antitumor Effects, in Part, by Modulating Mcl-1 in Human Oral Squamous Cell Carcinoma Cells. / Weng, Jing Ru; Bai, Li Yuan; Chiu, Shih Jiuan; Chiu, Chang Fang; Lin, Wei Yu; Hu, Jing Lan; Shieh, Tzong Ming.

於: Computational and Structural Biotechnology Journal, 卷 17, 01.01.2019, p. 151-159.

研究成果: 雜誌貢獻文章

Weng, Jing Ru ; Bai, Li Yuan ; Chiu, Shih Jiuan ; Chiu, Chang Fang ; Lin, Wei Yu ; Hu, Jing Lan ; Shieh, Tzong Ming. / Divaricoside Exerts Antitumor Effects, in Part, by Modulating Mcl-1 in Human Oral Squamous Cell Carcinoma Cells. 於: Computational and Structural Biotechnology Journal. 2019 ; 卷 17. 頁 151-159.
@article{86d0cef43c164441aefec9efe5e7fb84,
title = "Divaricoside Exerts Antitumor Effects, in Part, by Modulating Mcl-1 in Human Oral Squamous Cell Carcinoma Cells",
abstract = "Cardiac glycosides (CGs), prescribed to treat congestive heart failure and arrhythmias, exert potent antitumor activity. In this study, divaricoside (DIV), a CG isolated from Strophanthus divaricatus was examined for its antitumor potency in oral squamous cell carcinoma (OSCC) cells. Cell growth was inhibited by DIV in a dose- and time-dependent manner in SCC2095 and OECM-1 OSCC cells using MTT assays. DIV induced S and G2/M phase arrest accompanied by downregulation of phosphorylated CDC25C, CDC25C, and CDC2 in SCC2095 cells. In addition, DIV induced apoptosis by activating caspase-3 and downregulating the expression of Mcl-1. Furthermore, overexpression of Mcl-1 partially reversed DIV-induced death in SCC2095 cells. Additionally, western blot and transmission electron microscopy analyses also indicated that DIV induced autophagy in SCC2095 cells. However, the combination of autophagy inhibitor did not affect DIV-mediated apoptosis in SCC2095 cells. Together, these findings suggest that translational potential of DIV to be developed as a therapeutic agent for OSCC treatment.",
keywords = "Apoptosis, Autophagy, Cardiac glycoside, Divaricoside, Mcl-1, Oral squamous cell carcinoma",
author = "Weng, {Jing Ru} and Bai, {Li Yuan} and Chiu, {Shih Jiuan} and Chiu, {Chang Fang} and Lin, {Wei Yu} and Hu, {Jing Lan} and Shieh, {Tzong Ming}",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.csbj.2019.01.004",
language = "English",
volume = "17",
pages = "151--159",
journal = "Computational and Structural Biotechnology Journal",
issn = "2001-0370",
publisher = "Research Network of Computational and Structural Biotechnology",

}

TY - JOUR

T1 - Divaricoside Exerts Antitumor Effects, in Part, by Modulating Mcl-1 in Human Oral Squamous Cell Carcinoma Cells

AU - Weng, Jing Ru

AU - Bai, Li Yuan

AU - Chiu, Shih Jiuan

AU - Chiu, Chang Fang

AU - Lin, Wei Yu

AU - Hu, Jing Lan

AU - Shieh, Tzong Ming

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Cardiac glycosides (CGs), prescribed to treat congestive heart failure and arrhythmias, exert potent antitumor activity. In this study, divaricoside (DIV), a CG isolated from Strophanthus divaricatus was examined for its antitumor potency in oral squamous cell carcinoma (OSCC) cells. Cell growth was inhibited by DIV in a dose- and time-dependent manner in SCC2095 and OECM-1 OSCC cells using MTT assays. DIV induced S and G2/M phase arrest accompanied by downregulation of phosphorylated CDC25C, CDC25C, and CDC2 in SCC2095 cells. In addition, DIV induced apoptosis by activating caspase-3 and downregulating the expression of Mcl-1. Furthermore, overexpression of Mcl-1 partially reversed DIV-induced death in SCC2095 cells. Additionally, western blot and transmission electron microscopy analyses also indicated that DIV induced autophagy in SCC2095 cells. However, the combination of autophagy inhibitor did not affect DIV-mediated apoptosis in SCC2095 cells. Together, these findings suggest that translational potential of DIV to be developed as a therapeutic agent for OSCC treatment.

AB - Cardiac glycosides (CGs), prescribed to treat congestive heart failure and arrhythmias, exert potent antitumor activity. In this study, divaricoside (DIV), a CG isolated from Strophanthus divaricatus was examined for its antitumor potency in oral squamous cell carcinoma (OSCC) cells. Cell growth was inhibited by DIV in a dose- and time-dependent manner in SCC2095 and OECM-1 OSCC cells using MTT assays. DIV induced S and G2/M phase arrest accompanied by downregulation of phosphorylated CDC25C, CDC25C, and CDC2 in SCC2095 cells. In addition, DIV induced apoptosis by activating caspase-3 and downregulating the expression of Mcl-1. Furthermore, overexpression of Mcl-1 partially reversed DIV-induced death in SCC2095 cells. Additionally, western blot and transmission electron microscopy analyses also indicated that DIV induced autophagy in SCC2095 cells. However, the combination of autophagy inhibitor did not affect DIV-mediated apoptosis in SCC2095 cells. Together, these findings suggest that translational potential of DIV to be developed as a therapeutic agent for OSCC treatment.

KW - Apoptosis

KW - Autophagy

KW - Cardiac glycoside

KW - Divaricoside

KW - Mcl-1

KW - Oral squamous cell carcinoma

UR - http://www.scopus.com/inward/record.url?scp=85061099547&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061099547&partnerID=8YFLogxK

U2 - 10.1016/j.csbj.2019.01.004

DO - 10.1016/j.csbj.2019.01.004

M3 - Article

AN - SCOPUS:85061099547

VL - 17

SP - 151

EP - 159

JO - Computational and Structural Biotechnology Journal

JF - Computational and Structural Biotechnology Journal

SN - 2001-0370

ER -