Disulfide pairings and secondary structure of porcine β-microseminoprotein

Iren Wang, Tsun Ai Yu, Shih Hsiung Wu, Wen Chang Chang, Chinpan Chen

研究成果: 雜誌貢獻文章同行評審

11 引文 斯高帕斯(Scopus)

摘要

A sperm motility inhibitor isolated from porcine seminal plasma is identical to porcine β-microseminoprotein (MSP). Circular dichroism (CD) and nuclear magnetic resonance (NMR) data showed that the native and recombinant porcine MSPs exhibit very similar structure. The five disulfide pairings on porcine MSP were unambiguously assigned based on NMR data and further confirmed using structural calculations. Surprisingly, our derived pairings differ from those recently reported for ostrich MSP based on matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) analysis. Furthermore, the secondary structure was determined to comprise one four-stranded and two double-stranded antiparallel β-sheets. As we know, this is the first detailed secondary structure reported among several types of MSPs.
原文英語
頁(從 - 到)80-84
頁數5
期刊FEBS Letters
541
發行號1-3
DOIs
出版狀態已發佈 - 四月 24 2003
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 結構生物學
  • 生物化學
  • 分子生物學
  • 遺傳學
  • 細胞生物學

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