TY - JOUR
T1 - Disulfide pairings and secondary structure of porcine β-microseminoprotein
AU - Wang, Iren
AU - Yu, Tsun Ai
AU - Wu, Shih Hsiung
AU - Chang, Wen Chang
AU - Chen, Chinpan
PY - 2003/4/24
Y1 - 2003/4/24
N2 - A sperm motility inhibitor isolated from porcine seminal plasma is identical to porcine β-microseminoprotein (MSP). Circular dichroism (CD) and nuclear magnetic resonance (NMR) data showed that the native and recombinant porcine MSPs exhibit very similar structure. The five disulfide pairings on porcine MSP were unambiguously assigned based on NMR data and further confirmed using structural calculations. Surprisingly, our derived pairings differ from those recently reported for ostrich MSP based on matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) analysis. Furthermore, the secondary structure was determined to comprise one four-stranded and two double-stranded antiparallel β-sheets. As we know, this is the first detailed secondary structure reported among several types of MSPs.
AB - A sperm motility inhibitor isolated from porcine seminal plasma is identical to porcine β-microseminoprotein (MSP). Circular dichroism (CD) and nuclear magnetic resonance (NMR) data showed that the native and recombinant porcine MSPs exhibit very similar structure. The five disulfide pairings on porcine MSP were unambiguously assigned based on NMR data and further confirmed using structural calculations. Surprisingly, our derived pairings differ from those recently reported for ostrich MSP based on matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) analysis. Furthermore, the secondary structure was determined to comprise one four-stranded and two double-stranded antiparallel β-sheets. As we know, this is the first detailed secondary structure reported among several types of MSPs.
KW - Human prostate secretory protein of 94 amino acids
KW - Na,K-adenosine triphosphatase inhibitor
KW - Nuclear magnetic resonance
KW - Structure
KW - β-Microseminoprotein
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U2 - 10.1016/S0014-5793(03)00308-9
DO - 10.1016/S0014-5793(03)00308-9
M3 - Article
C2 - 12706823
AN - SCOPUS:0037464493
VL - 541
SP - 80
EP - 84
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-3
ER -