Distinctive sodium and calcium regulation associated with sex differences in atrial electrophysiology of rabbits

Wen Chin Tsai, Yao Chang Chen, Yu Hsun Kao, Yen Yu Lu, Shih Ann Chen, Yi Jen Chen

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17 引文 斯高帕斯(Scopus)

摘要

Background Sex and sodium/calcium regulation play critical roles in cardiac electrophysiology and atrial arrhythmogenesis. We investigated whether sodium and calcium contributed to sex differences in atrial electrophysiology. Methods Whole-cell patch clamp techniques and the indo-1 fluorometric ratio technique were used to investigate the ionic current and intracellular calcium in single isolated male and female rabbit myocytes from the left atrium posterior wall (LAPW) and right atrium (RA). Results Female LAPW (n = 95) and RA (n = 49) myocytes had larger cell widths (15.1 ± 0.4 vs. 13.8 ± 0.4 μm, p <0.05; 14.9 ± 0.6 vs. 13.5 ± 0.4 μm, p <0.05) than male LAPW (n = 142) and RA (n = 57) myocytes. Male LAPW myocytes (n = 26) had a higher incidence (57 vs. 16%, p <0.05) of delayed afterdepolarizations (DADs) than female LAPW myocytes (n = 24) but there were similar incidences (20 vs. 20%, p > 0.05) of DADs in male and female RA myocytes. The late sodium current, calcium transients, and sarcoplasmic reticulum calcium contents were larger in male than female LAPW myocytes but were similar in male and female RA myocytes. However, the ICa-L and nickel-sensitive sodium/calcium exchanger currents were similar between two groups. Different from those in female myocytes, ouabain (10 μM) only induced repeated atrial beats (0 to 45%, p <0.05) in male myocytes (n = 11). Moreover, ranolazine (3 μM) perfusion (4.5 ± 0.6 vs. 1 min, p <0.05) was required to decrease the amplitude of DADs in male but not female LAPW myocytes. Conclusions Increased late sodium currents and calcium contents may contribute to higher arrhythmogenesis in male LAPW myocytes.

原文英語
頁(從 - 到)4658-4666
頁數9
期刊International Journal of Cardiology
168
發行號5
DOIs
出版狀態已發佈 - 十月 12 2013

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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