Distinct regulation of cyclooxygenase-2 by interleukin-1β in normal and endometriotic stromal cells

Meng Hsing Wu, Chu An Wang, Chen Chung Lin, Lei Chin Chen, Wen Chang Chang, Shaw Jenq Tsai

研究成果: 雜誌貢獻文章同行評審

117 引文 斯高帕斯(Scopus)


Aberrant production of cyclooxygenase-2 (COX-2) plays pivotal roles in many pathological processes including tumorigenesis and endometriosis, although the underlying mechanism remains obscure. Herein we report evidence to demonstrate that COX-2 is distinctly regulated by IL-1β in normal and endomeiriotic stroma. Ectopic endometriotic stromal cell is at least 100 times more sensitive to IL-1β treatment, compared with its eutopic counterpart. Induction of COX-2 expression in normal endometrial stroma by IL-1β is primary due to enhancement of COX-2 mRNA stability. In contrast, IL-1β not only increases COX-2 mRNA stability but also up-regulates COX-2 promoter activity in eciopic endometriotic stroma. Induction of COX-2 promoter activity by IL-1β is mediated via MAPK-dependent phosphorylation of cAMP-responding element binding protein. Promoter activity and EMSAs demonstrate that a cAMP response element site located at -571/-564 of COX-2 promoter is critical for IL-1β-induced COX-2 gene expression. Our results indicate that elevation of COX-2 expression in endometriotic tissues may result from increased sensitivity to proinflammatory cytokines such as IL-1β, which is consistently present in the peritoneal fluid of endometriosis patients. Distinct regulation of COX-2 gene by IL-1β may play a critical role in pathopliysiological processes such as cancer formation and endometriosis.

頁(從 - 到)286-295
期刊Journal of Clinical Endocrinology and Metabolism
出版狀態已發佈 - 2005

ASJC Scopus subject areas

  • 內分泌學、糖尿病和代謝
  • 生物化學
  • 內分泌
  • 臨床生物化學
  • 生物化學(醫學)


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