Distinct functions of integrin α and β subunit cytoplasmic domains in cell spreading and formation of focal adhesions

J. Ylänne, Y. Chen, T. E. O'Toole, J. C. Loftus, Y. Takada, M. H. Ginsberg

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190 引文 斯高帕斯(Scopus)

摘要

Integrin-mediated cell adhesion often results in cell spreading and the formation of focal adhesions. We exploited the capacity of recombinant human αIIbβ3 integrin to endow heterologous cells with the ability to adhere and spread on fibrinogen to study the role of integrin cytoplasmic domains in initiation of cell spreading and focal adhesions. The same constructs were also used to analyze the role of the cytoplasmic domains in maintenance of the fidelity of the integrin repertoire at focal adhesions. Truncation mutants of the cytoplasmic domain of αIIb did not interfere with the ability of αIIbβ3 to initiate cell spreading and form focal adhesions. Nevertheless, deletion of the αIIb cytoplasmic domain allowed indiscriminate recruitment of αIIbβ3 to focal adhesions formed by other integrins. Truncation of the ft subunit cytoplasmic domain abolished cell spreading mediated by αIIbβ3 and also abrogated recruitment of αIIbβ3 to focal adhesions. This truncation also dramatically impaired the ability of αIIbβ3 to mediate the contraction of fibrin gels. In contrast, the β3 subunit cytoplasmic truncation did not reduce the fibrinogen binding affinity of αIIbβ3. Thus, the integrin β3 subunit cytoplasmic domain is necessary and sufficient for initiation of cell spreading and focal adhesion formation. Further, the β3 cytoplasmic domain is required for the transmission of intracellular contractile forces to fibrin gels. The α subunit cytoplasmic domain maintains the fidelity of recruitment of the integrins to focal adhesions and thus regulates their repertoire of integrins.

原文英語
頁(從 - 到)223-233
頁數11
期刊Journal of Cell Biology
122
發行號1
DOIs
出版狀態已發佈 - 七月 1993

ASJC Scopus subject areas

  • Cell Biology

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