Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies

Ta Chun Cheng, Chu Hsiang Pan, Chien Shu Chen, Kuo Hsiang Chuang, Chih Hung Chuang, Chien Chaio Huang, Yu Yi Chu, Ya Chun Yang, Pei Yu Chu, Chien Han Kao, Yuan Chin Hsieh, Tian Lu Cheng

研究成果: 雜誌貢獻文章

4 引文 斯高帕斯(Scopus)

摘要

The envelope glycoprotein E2 of classical swine fever virus (CSFV) is widely used as a marker for measuring vaccine efficacy and antibody titer. The glycosylation profile of E2 may affect the immunogenicity of the vaccine and the timing of re-vaccination. In this study, a human embryonic kidney cell line was used to secrete fully-glycosylated CSFV E2, which was then coated onto ELISA plates without purification or adjustment.The resulting E2-secreting medium-direct-coating (E2-mDc) ELISA was successfully used to measure anti-E2 antibody titers in vaccinated and field pig sera samples. Compared with a virus neutralization test (as standard), the E2-mDc ELISA was found to be more accurate (90%) than a commercial CSFV antibody diagnostic kit (62%). In conclusion, the mammalian cell-secreted antigen can provide cheap, accurate and effective assays for vaccine efficacy and disease diagnoses.

原文英語
頁(從 - 到)107-109
頁數3
期刊Veterinary Journal
205
發行號1
DOIs
出版狀態已發佈 - 七月 1 2015

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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    Cheng, T. C., Pan, C. H., Chen, C. S., Chuang, K. H., Chuang, C. H., Huang, C. C., Chu, Y. Y., Yang, Y. C., Chu, P. Y., Kao, C. H., Hsieh, Y. C., & Cheng, T. L. (2015). Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies. Veterinary Journal, 205(1), 107-109. https://doi.org/10.1016/j.tvjl.2015.02.007