Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity

Ming Hsien Chien, Tsung Ho Ying, Yih Shou Hsieh, Yu Chao Chang, Chia Ming Yeh, Jiunn Liang Ko, Wen Sen Lee, Jer Hua Chang, Shun Fa Yang

研究成果: 雜誌貢獻 › 文章

49 引文 (Scopus)

摘要

Oral cancer mortality has increased during the last decade due to the difficulties in treating related metastasis. Dioscorea nipponica Makino, a popular folk medicine, exerts anti-obesity and anti-inflammation properties. However, the effect of this folk medicine on metastasis of oral cancer has yet to be fully elucidated. The present study demonstrates that D. nipponica extracts (DNE), at a range of concentrations (0-50 μg/mL), concentration-dependently inhibited migration/invasion capacities of human oral cancer cells, HSC-3, without cytotoxic effects. The anti-migration effect of DNE was also observed in two other OSCC cell lines, Ca9-22 and Cal-27. Zymography, real time PCR, and Western blotting analyses revealed that DNE inhibited matrix metalloproteinase-2 (MMP-2) enzyme activity, and RNA and protein expression. The inhibitory effects of DNE on MMP-2 proceeded by up-regulating tissue inhibitor of metalloproteinase-2 (TIMP-2), as well as suppressing nuclear translocation and DNA binding activity of cAMP response element-binding (CREB) and activating protein-1 (AP-1) on the MMP-2 promoter in HSC-3 cells. In conclusion, DNE inhibited the invasion of oral cancer cells and may have potential use as a chemopreventive agent against oral cancer metastasis.
原文英語
頁（從 - 到）558-566
頁數9
期刊Food and Chemical Toxicology
卷50
發行號3-4
DOIs
出版狀態已發佈 - 三月 2012

指紋

Dioscorea nipponica
Dioscorea
Cyclic AMP Response Element-Binding Protein
gelatinase A
response elements
Mouth Neoplasms
Matrix Metalloproteinase 2
Response Elements
Cells
Modulation
Medicine
metastasis
extracts
Traditional Medicine
Tissue Inhibitor of Metalloproteinase-2
Neoplasm Metastasis
Proteins
proteins
Enzyme activity
cells

ASJC Scopus subject areas

  • Food Science
  • Toxicology

引用此文

Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity. / Chien, Ming Hsien; Ying, Tsung Ho; Hsieh, Yih Shou; Chang, Yu Chao; Yeh, Chia Ming; Ko, Jiunn Liang; Lee, Wen Sen; Chang, Jer Hua; Yang, Shun Fa.

於: Food and Chemical Toxicology, 卷 50, 編號 3-4, 03.2012, p. 558-566.

研究成果: 雜誌貢獻 › 文章

Chien, MH, Ying, TH, Hsieh, YS, Chang, YC, Yeh, CM, Ko, JL, Lee, WS, Chang, JH & Yang, SF 2012, 'Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity', Food and Chemical Toxicology, 卷 50, 編號 3-4, 頁 558-566. https://doi.org/10.1016/j.fct.2011.12.016
Chien MH, Ying TH, Hsieh YS, Chang YC, Yeh CM, Ko JL 等. Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity. Food and Chemical Toxicology. 2012 3月;50(3-4):558-566. https://doi.org/10.1016/j.fct.2011.12.016
Chien, Ming Hsien ; Ying, Tsung Ho ; Hsieh, Yih Shou ; Chang, Yu Chao ; Yeh, Chia Ming ; Ko, Jiunn Liang ; Lee, Wen Sen ; Chang, Jer Hua ; Yang, Shun Fa. / Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity. 於: Food and Chemical Toxicology. 2012 ; 卷 50, 編號 3-4. 頁 558-566.
@article{d5f8b5f8340e418787beef265da445b9,
title = "Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity",
abstract = "Oral cancer mortality has increased during the last decade due to the difficulties in treating related metastasis. Dioscorea nipponica Makino, a popular folk medicine, exerts anti-obesity and anti-inflammation properties. However, the effect of this folk medicine on metastasis of oral cancer has yet to be fully elucidated. The present study demonstrates that D. nipponica extracts (DNE), at a range of concentrations (0-50 μg/mL), concentration-dependently inhibited migration/invasion capacities of human oral cancer cells, HSC-3, without cytotoxic effects. The anti-migration effect of DNE was also observed in two other OSCC cell lines, Ca9-22 and Cal-27. Zymography, real time PCR, and Western blotting analyses revealed that DNE inhibited matrix metalloproteinase-2 (MMP-2) enzyme activity, and RNA and protein expression. The inhibitory effects of DNE on MMP-2 proceeded by up-regulating tissue inhibitor of metalloproteinase-2 (TIMP-2), as well as suppressing nuclear translocation and DNA binding activity of cAMP response element-binding (CREB) and activating protein-1 (AP-1) on the MMP-2 promoter in HSC-3 cells. In conclusion, DNE inhibited the invasion of oral cancer cells and may have potential use as a chemopreventive agent against oral cancer metastasis.",
keywords = "Dioscorea nipponica, Invasion, Migration, MMP-2, Oral cancer",
author = "Chien, {Ming Hsien} and Ying, {Tsung Ho} and Hsieh, {Yih Shou} and Chang, {Yu Chao} and Yeh, {Chia Ming} and Ko, {Jiunn Liang} and Lee, {Wen Sen} and Chang, {Jer Hua} and Yang, {Shun Fa}",
year = "2012",
month = "3",
doi = "10.1016/j.fct.2011.12.016",
language = "English",
volume = "50",
pages = "558--566",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",
publisher = "Elsevier Limited",
number = "3-4",

}

TY - JOUR

T1 - Dioscorea nipponica Makino inhibits migration and invasion of human oral cancer HSC-3 cells by transcriptional inhibition of matrix metalloproteinase-2 through modulation of CREB and AP-1 activity

AU - Chien, Ming Hsien

AU - Ying, Tsung Ho

AU - Hsieh, Yih Shou

AU - Chang, Yu Chao

AU - Yeh, Chia Ming

AU - Ko, Jiunn Liang

AU - Lee, Wen Sen

AU - Chang, Jer Hua

AU - Yang, Shun Fa

PY - 2012/3

Y1 - 2012/3

N2 - Oral cancer mortality has increased during the last decade due to the difficulties in treating related metastasis. Dioscorea nipponica Makino, a popular folk medicine, exerts anti-obesity and anti-inflammation properties. However, the effect of this folk medicine on metastasis of oral cancer has yet to be fully elucidated. The present study demonstrates that D. nipponica extracts (DNE), at a range of concentrations (0-50 μg/mL), concentration-dependently inhibited migration/invasion capacities of human oral cancer cells, HSC-3, without cytotoxic effects. The anti-migration effect of DNE was also observed in two other OSCC cell lines, Ca9-22 and Cal-27. Zymography, real time PCR, and Western blotting analyses revealed that DNE inhibited matrix metalloproteinase-2 (MMP-2) enzyme activity, and RNA and protein expression. The inhibitory effects of DNE on MMP-2 proceeded by up-regulating tissue inhibitor of metalloproteinase-2 (TIMP-2), as well as suppressing nuclear translocation and DNA binding activity of cAMP response element-binding (CREB) and activating protein-1 (AP-1) on the MMP-2 promoter in HSC-3 cells. In conclusion, DNE inhibited the invasion of oral cancer cells and may have potential use as a chemopreventive agent against oral cancer metastasis.

AB - Oral cancer mortality has increased during the last decade due to the difficulties in treating related metastasis. Dioscorea nipponica Makino, a popular folk medicine, exerts anti-obesity and anti-inflammation properties. However, the effect of this folk medicine on metastasis of oral cancer has yet to be fully elucidated. The present study demonstrates that D. nipponica extracts (DNE), at a range of concentrations (0-50 μg/mL), concentration-dependently inhibited migration/invasion capacities of human oral cancer cells, HSC-3, without cytotoxic effects. The anti-migration effect of DNE was also observed in two other OSCC cell lines, Ca9-22 and Cal-27. Zymography, real time PCR, and Western blotting analyses revealed that DNE inhibited matrix metalloproteinase-2 (MMP-2) enzyme activity, and RNA and protein expression. The inhibitory effects of DNE on MMP-2 proceeded by up-regulating tissue inhibitor of metalloproteinase-2 (TIMP-2), as well as suppressing nuclear translocation and DNA binding activity of cAMP response element-binding (CREB) and activating protein-1 (AP-1) on the MMP-2 promoter in HSC-3 cells. In conclusion, DNE inhibited the invasion of oral cancer cells and may have potential use as a chemopreventive agent against oral cancer metastasis.

KW - Dioscorea nipponica

KW - Invasion

KW - Migration

KW - MMP-2

KW - Oral cancer

UR - http://www.scopus.com/inward/record.url?scp=84863261589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863261589&partnerID=8YFLogxK

U2 - 10.1016/j.fct.2011.12.016

DO - 10.1016/j.fct.2011.12.016

M3 - Article

C2 - 22210353

AN - SCOPUS:84863261589

VL - 50

SP - 558

EP - 566

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

IS - 3-4

ER -

存取文件