Differentiation of pheochromocytoma PC 12 cells induced by human urine extract and the involvement of the extracellular signal-regulated kinase signaling pathway

Chih Jung Yao, Gi Ming Lai, Chin Feng Chan, Ya Yu Yang, Frank C. Liu, Shuang En Chuang

研究成果: 雜誌貢獻文章

11 引文 斯高帕斯(Scopus)

摘要

Objective: The aim of this study was to investigate the effects of a human urine preparation on the differentiation of tumor cells. Design: The pheochromocytoma PC12 cells were used to examine the effects of a human urine preparation, CDA-2 on the induction of differentiation markers, neurofilaments, and compared with that induced by nerve growth factor (NGF). The MAPK/ERK kinase (MEK) inhibitor U0126 was used to examine the involvement of mitogen-activated protein kinase (MAPK) signaling pathway in this differentiation inducing effect. Results: We find that CDA-2 could induce differentiation of pheochromocytoma PC12 cells, as evidenced by the markedly increased expression of neurofilaments to a level comparable to those induced by NGF. This phenomenon was accompanied by the phosphorylation of extracellular-signal-regulated kinase (ERK) and could be inhibited by the MEK inhibitor, U0126. Conclusions: Our results demonstrate the presence of active components in the human urine extract that can induce differentiation of PC12 cells and may involve the ERK signaling pathway. This may provide new insights for seeking novel differentiation agents and offer hope for cancer patients.
原文英語
頁(從 - 到)903-908
頁數6
期刊Journal of Alternative and Complementary Medicine
11
發行號5
DOIs
出版狀態已發佈 - 十月 2005
對外發佈Yes

ASJC Scopus subject areas

  • Medicine(all)
  • Complementary and alternative medicine
  • Nursing(all)

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