Differentiation effects of platelet-rich plasma concentrations on synovial fluid mesenchymal stem cells from pigs cultivated in alginate complex hydrogel

Hao Che Tang, Wei Chuan Chen, Chih Wei Chiang, Lei Yen Chen, Yu Ching Chang, Chih Hwa Chen

研究成果: 雜誌貢獻文章

12 引文 (Scopus)

摘要

This article studied the effects of platelet-rich plasma (PRP) on the potential of synovial fluid mesenchymal stem cells (SF-MSCs) to differentiate. The PRP and SF-MSCs were obtained from the blood and knees of pigs, respectively. The identification of SF-MSCs and their ability to differentiate were studied by histological and surface epitopes, respectively. The SF-MSCs can undergo trilineage mesenchymal differentiation under osteogenic, chondrogenic, and adipocyte induction. The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation. A 50% PRP concentration yielded better differentiation than the 20% PRP concentration. PRP favored the chondrogenesis of SF-MSCs over their osteogenesis in a manner that depended on the ratios of type II collagen/type I collagen and aggrecan/osteopontin. Eventually, PRP promoted the proliferation of SF-MSCs and induced chondrogenic differentiation of SF-MSCs in vitro. Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.

原文英語
頁(從 - 到)18507-18521
頁數15
期刊International Journal of Molecular Sciences
16
發行號8
DOIs
出版狀態已發佈 - 八月 7 2015

指紋

Platelet-Rich Plasma
swine
stem cells
Hydrogel
Synovial Fluid
Alginate
Platelets
Stem cells
platelets
Mesenchymal Stromal Cells
Hydrogels
Swine
Plasmas
Fluids
fluids
collagens
Collagen
osteogenesis
Epitopes
alginic acid

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications

引用此文

Differentiation effects of platelet-rich plasma concentrations on synovial fluid mesenchymal stem cells from pigs cultivated in alginate complex hydrogel. / Tang, Hao Che; Chen, Wei Chuan; Chiang, Chih Wei; Chen, Lei Yen; Chang, Yu Ching; Chen, Chih Hwa.

於: International Journal of Molecular Sciences, 卷 16, 編號 8, 07.08.2015, p. 18507-18521.

研究成果: 雜誌貢獻文章

@article{dd259d0c04ad4caabe8c0d2ad8006e68,
title = "Differentiation effects of platelet-rich plasma concentrations on synovial fluid mesenchymal stem cells from pigs cultivated in alginate complex hydrogel",
abstract = "This article studied the effects of platelet-rich plasma (PRP) on the potential of synovial fluid mesenchymal stem cells (SF-MSCs) to differentiate. The PRP and SF-MSCs were obtained from the blood and knees of pigs, respectively. The identification of SF-MSCs and their ability to differentiate were studied by histological and surface epitopes, respectively. The SF-MSCs can undergo trilineage mesenchymal differentiation under osteogenic, chondrogenic, and adipocyte induction. The effects of various PRP concentrations (0{\%}, 20{\%} and 50{\%} PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation. A 50{\%} PRP concentration yielded better differentiation than the 20{\%} PRP concentration. PRP favored the chondrogenesis of SF-MSCs over their osteogenesis in a manner that depended on the ratios of type II collagen/type I collagen and aggrecan/osteopontin. Eventually, PRP promoted the proliferation of SF-MSCs and induced chondrogenic differentiation of SF-MSCs in vitro. Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.",
keywords = "Alginate hydrogel, Chondrogenesis, DNA proliferation, Gene expressions, Pigs, Platelet-rich plasma, Synovial fluid mesenchymal stem cells",
author = "Tang, {Hao Che} and Chen, {Wei Chuan} and Chiang, {Chih Wei} and Chen, {Lei Yen} and Chang, {Yu Ching} and Chen, {Chih Hwa}",
year = "2015",
month = "8",
day = "7",
doi = "10.3390/ijms160818507",
language = "English",
volume = "16",
pages = "18507--18521",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "MDPI AG",
number = "8",

}

TY - JOUR

T1 - Differentiation effects of platelet-rich plasma concentrations on synovial fluid mesenchymal stem cells from pigs cultivated in alginate complex hydrogel

AU - Tang, Hao Che

AU - Chen, Wei Chuan

AU - Chiang, Chih Wei

AU - Chen, Lei Yen

AU - Chang, Yu Ching

AU - Chen, Chih Hwa

PY - 2015/8/7

Y1 - 2015/8/7

N2 - This article studied the effects of platelet-rich plasma (PRP) on the potential of synovial fluid mesenchymal stem cells (SF-MSCs) to differentiate. The PRP and SF-MSCs were obtained from the blood and knees of pigs, respectively. The identification of SF-MSCs and their ability to differentiate were studied by histological and surface epitopes, respectively. The SF-MSCs can undergo trilineage mesenchymal differentiation under osteogenic, chondrogenic, and adipocyte induction. The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation. A 50% PRP concentration yielded better differentiation than the 20% PRP concentration. PRP favored the chondrogenesis of SF-MSCs over their osteogenesis in a manner that depended on the ratios of type II collagen/type I collagen and aggrecan/osteopontin. Eventually, PRP promoted the proliferation of SF-MSCs and induced chondrogenic differentiation of SF-MSCs in vitro. Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.

AB - This article studied the effects of platelet-rich plasma (PRP) on the potential of synovial fluid mesenchymal stem cells (SF-MSCs) to differentiate. The PRP and SF-MSCs were obtained from the blood and knees of pigs, respectively. The identification of SF-MSCs and their ability to differentiate were studied by histological and surface epitopes, respectively. The SF-MSCs can undergo trilineage mesenchymal differentiation under osteogenic, chondrogenic, and adipocyte induction. The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation. A 50% PRP concentration yielded better differentiation than the 20% PRP concentration. PRP favored the chondrogenesis of SF-MSCs over their osteogenesis in a manner that depended on the ratios of type II collagen/type I collagen and aggrecan/osteopontin. Eventually, PRP promoted the proliferation of SF-MSCs and induced chondrogenic differentiation of SF-MSCs in vitro. Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.

KW - Alginate hydrogel

KW - Chondrogenesis

KW - DNA proliferation

KW - Gene expressions

KW - Pigs

KW - Platelet-rich plasma

KW - Synovial fluid mesenchymal stem cells

UR - http://www.scopus.com/inward/record.url?scp=84938858229&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938858229&partnerID=8YFLogxK

U2 - 10.3390/ijms160818507

DO - 10.3390/ijms160818507

M3 - Article

C2 - 26262616

AN - SCOPUS:84938858229

VL - 16

SP - 18507

EP - 18521

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 8

ER -