Differential viral loads of human papillomavirus 16 and 58 infections in the spectrum of cervical carcinogenesis

H. C. Lai, M. Y. Peng, S. Nieh, C. P. Yu, C. C. Chang, Y. W. Lin, C. A. Sun, T. Y. Chu

研究成果: 雜誌貢獻文章

13 引文 (Scopus)

摘要

Human papillomavirus (HPV) load was reported to be related to the severity of cervical neoplasia but with controversy. The viral load-disease severity relationship was showed in HPV 16, but no study was made in HPV 58, the second most prevalent HPV in cervical cancer in East Asia. We studied cervical HPV loads in HPV 16- and HPV 58-infected cases of normal, low-grade squamous intraepithelial lesions (LSIL), high-grade squamous intraepithelial lesions (HSIL), and invasive cervical cancer (CC) by using quantitative polymerase chain reaction (Q-PCR) with type-specific primers in defined cell number. With the exception of HPV 16 infection in normal, viral loads varied greatly in each disease regardless of genotypes. The load of HPV 16 differed significantly among disease severities, with a dramatic increase from normal (1.14 ± 2.25 copies/cell) to LSIL, HSIL, and CC (1599 ± 2301, 7489 ± 24,087 and 1878 ± 2979 copies/cell, respectively) (P <0.01). No significant difference was noted among different HPV 58 infections, with loads in normal, LSIL, HSIL, and CC of 503 ± 641, 7951 ± 27,557, 353 ± 744, and 1139 ± 2895 copies/cell, respectively. In comparison with HPV 16, HPV 58 subclinical infection confers a significant higher load (P <0.01). Different HPV types behave differentially in the spectrum of cervical carcinogenesis. Unlike HPV 16, the infection load of HPV 58 does not correlate to the clinical severity. The wide variation of HPV loads among different HPV types and among squamous intraepithelial lesions and CC makes the viral load test unrealistic in differentiating different severities of cervical neoplasia.

原文英語
頁(從 - 到)730-735
頁數6
期刊International Journal of Gynecological Cancer
16
發行號2
DOIs
出版狀態已發佈 - 三月 2006
對外發佈Yes

指紋

Human papillomavirus 16
Viral Load
Carcinogenesis
Infection
Uterine Cervical Neoplasms
Papillomavirus Infections
Asymptomatic Infections
Far East
Virus Diseases
Squamous Intraepithelial Lesions of the Cervix
Neoplasms
Cell Count
Genotype

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology
  • Cancer Research

引用此文

Differential viral loads of human papillomavirus 16 and 58 infections in the spectrum of cervical carcinogenesis. / Lai, H. C.; Peng, M. Y.; Nieh, S.; Yu, C. P.; Chang, C. C.; Lin, Y. W.; Sun, C. A.; Chu, T. Y.

於: International Journal of Gynecological Cancer, 卷 16, 編號 2, 03.2006, p. 730-735.

研究成果: 雜誌貢獻文章

Lai, H. C. ; Peng, M. Y. ; Nieh, S. ; Yu, C. P. ; Chang, C. C. ; Lin, Y. W. ; Sun, C. A. ; Chu, T. Y. / Differential viral loads of human papillomavirus 16 and 58 infections in the spectrum of cervical carcinogenesis. 於: International Journal of Gynecological Cancer. 2006 ; 卷 16, 編號 2. 頁 730-735.
@article{613137c020df43349043402a6db90fc2,
title = "Differential viral loads of human papillomavirus 16 and 58 infections in the spectrum of cervical carcinogenesis",
abstract = "Human papillomavirus (HPV) load was reported to be related to the severity of cervical neoplasia but with controversy. The viral load-disease severity relationship was showed in HPV 16, but no study was made in HPV 58, the second most prevalent HPV in cervical cancer in East Asia. We studied cervical HPV loads in HPV 16- and HPV 58-infected cases of normal, low-grade squamous intraepithelial lesions (LSIL), high-grade squamous intraepithelial lesions (HSIL), and invasive cervical cancer (CC) by using quantitative polymerase chain reaction (Q-PCR) with type-specific primers in defined cell number. With the exception of HPV 16 infection in normal, viral loads varied greatly in each disease regardless of genotypes. The load of HPV 16 differed significantly among disease severities, with a dramatic increase from normal (1.14 ± 2.25 copies/cell) to LSIL, HSIL, and CC (1599 ± 2301, 7489 ± 24,087 and 1878 ± 2979 copies/cell, respectively) (P <0.01). No significant difference was noted among different HPV 58 infections, with loads in normal, LSIL, HSIL, and CC of 503 ± 641, 7951 ± 27,557, 353 ± 744, and 1139 ± 2895 copies/cell, respectively. In comparison with HPV 16, HPV 58 subclinical infection confers a significant higher load (P <0.01). Different HPV types behave differentially in the spectrum of cervical carcinogenesis. Unlike HPV 16, the infection load of HPV 58 does not correlate to the clinical severity. The wide variation of HPV loads among different HPV types and among squamous intraepithelial lesions and CC makes the viral load test unrealistic in differentiating different severities of cervical neoplasia.",
keywords = "Cervical cancer, HPV, Squamous intraepithelial lesion, Viral load",
author = "Lai, {H. C.} and Peng, {M. Y.} and S. Nieh and Yu, {C. P.} and Chang, {C. C.} and Lin, {Y. W.} and Sun, {C. A.} and Chu, {T. Y.}",
year = "2006",
month = "3",
doi = "10.1111/j.1525-1438.2006.00390.x",
language = "English",
volume = "16",
pages = "730--735",
journal = "International Journal of Gynecological Cancer",
issn = "1048-891X",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Differential viral loads of human papillomavirus 16 and 58 infections in the spectrum of cervical carcinogenesis

AU - Lai, H. C.

AU - Peng, M. Y.

AU - Nieh, S.

AU - Yu, C. P.

AU - Chang, C. C.

AU - Lin, Y. W.

AU - Sun, C. A.

AU - Chu, T. Y.

PY - 2006/3

Y1 - 2006/3

N2 - Human papillomavirus (HPV) load was reported to be related to the severity of cervical neoplasia but with controversy. The viral load-disease severity relationship was showed in HPV 16, but no study was made in HPV 58, the second most prevalent HPV in cervical cancer in East Asia. We studied cervical HPV loads in HPV 16- and HPV 58-infected cases of normal, low-grade squamous intraepithelial lesions (LSIL), high-grade squamous intraepithelial lesions (HSIL), and invasive cervical cancer (CC) by using quantitative polymerase chain reaction (Q-PCR) with type-specific primers in defined cell number. With the exception of HPV 16 infection in normal, viral loads varied greatly in each disease regardless of genotypes. The load of HPV 16 differed significantly among disease severities, with a dramatic increase from normal (1.14 ± 2.25 copies/cell) to LSIL, HSIL, and CC (1599 ± 2301, 7489 ± 24,087 and 1878 ± 2979 copies/cell, respectively) (P <0.01). No significant difference was noted among different HPV 58 infections, with loads in normal, LSIL, HSIL, and CC of 503 ± 641, 7951 ± 27,557, 353 ± 744, and 1139 ± 2895 copies/cell, respectively. In comparison with HPV 16, HPV 58 subclinical infection confers a significant higher load (P <0.01). Different HPV types behave differentially in the spectrum of cervical carcinogenesis. Unlike HPV 16, the infection load of HPV 58 does not correlate to the clinical severity. The wide variation of HPV loads among different HPV types and among squamous intraepithelial lesions and CC makes the viral load test unrealistic in differentiating different severities of cervical neoplasia.

AB - Human papillomavirus (HPV) load was reported to be related to the severity of cervical neoplasia but with controversy. The viral load-disease severity relationship was showed in HPV 16, but no study was made in HPV 58, the second most prevalent HPV in cervical cancer in East Asia. We studied cervical HPV loads in HPV 16- and HPV 58-infected cases of normal, low-grade squamous intraepithelial lesions (LSIL), high-grade squamous intraepithelial lesions (HSIL), and invasive cervical cancer (CC) by using quantitative polymerase chain reaction (Q-PCR) with type-specific primers in defined cell number. With the exception of HPV 16 infection in normal, viral loads varied greatly in each disease regardless of genotypes. The load of HPV 16 differed significantly among disease severities, with a dramatic increase from normal (1.14 ± 2.25 copies/cell) to LSIL, HSIL, and CC (1599 ± 2301, 7489 ± 24,087 and 1878 ± 2979 copies/cell, respectively) (P <0.01). No significant difference was noted among different HPV 58 infections, with loads in normal, LSIL, HSIL, and CC of 503 ± 641, 7951 ± 27,557, 353 ± 744, and 1139 ± 2895 copies/cell, respectively. In comparison with HPV 16, HPV 58 subclinical infection confers a significant higher load (P <0.01). Different HPV types behave differentially in the spectrum of cervical carcinogenesis. Unlike HPV 16, the infection load of HPV 58 does not correlate to the clinical severity. The wide variation of HPV loads among different HPV types and among squamous intraepithelial lesions and CC makes the viral load test unrealistic in differentiating different severities of cervical neoplasia.

KW - Cervical cancer

KW - HPV

KW - Squamous intraepithelial lesion

KW - Viral load

UR - http://www.scopus.com/inward/record.url?scp=33646031219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646031219&partnerID=8YFLogxK

U2 - 10.1111/j.1525-1438.2006.00390.x

DO - 10.1111/j.1525-1438.2006.00390.x

M3 - Article

C2 - 16681753

AN - SCOPUS:33646031219

VL - 16

SP - 730

EP - 735

JO - International Journal of Gynecological Cancer

JF - International Journal of Gynecological Cancer

SN - 1048-891X

IS - 2

ER -