Differential expression of type 1 angiotensin II receptor mRNA and aldosterone responsiveness to angiotensin in aldosterone-producing adenoma

Yung Ming Chen, Kwan Dun Wu, Mi I. Hu-Tsai, Jan Show Chu, Ming Kuen Lai, Bor Shen Hsieh

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15 引文 斯高帕斯(Scopus)

摘要

Aldosterone secretion in most patients with aldosterone-producing adenomas (APAs) is typically unresponsive to angiotensin II stimulation (AII- unresponsive, AII-U). In some patients, however, plasma aldosterone increases in response to AII stimulation (All-responsive, AII-R). This differential aldosterone responsiveness could be related to the levels of type 1 AII receptors (AT1R) in the APA. To test this hypothesis, plasma aldosterone levels in response to upright posture and/or sequential high- and low-salt diets were measured by radioimmunoassay in nine patients with APAs. AT1R mRNA levels in the adenomas were quantified by competitive reverse transcription-polymerase chain reaction and correlated to the cellular composition of the adenoma. Two patients were categorised as AII-R by an increase of plasma aldosterone greater than 50% over the baseline. The remaining seven patients who had blunted plasma aldosterone responses were classified as AII-U. Histologically, the AII-R APAs consisted predominantly of zona glomerulosa (ZG)-like cells (> 90%), while the AII-U APAs contained zona fasciculata (ZF)-like cells ranging from 28 to 72%. There was an inverse relationship between the levels of AT1R mRNA in the APA and the percentage of ZF-like cells in the adenoma (n = 9, r = 0.73, P <0.05). In situ hybridisation findings demonstrated that AT R mRNA was more uniform and intensive in ZG-like cells than in ZF-like cells. These results suggest that heterogenous aldosterone responsiveness to angiotensin in APAs is histologically dependent and related to the differential expression of AT1R mRNA in the adenoma.

原文英語
頁(從 - 到)47-55
頁數9
期刊Molecular and Cellular Endocrinology
152
發行號1-2
DOIs
出版狀態已發佈 - 六月 25 1999
對外發佈

ASJC Scopus subject areas

  • 內分泌
  • 內分泌學、糖尿病和代謝

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