Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins

Hung I. Yeh, Yu J. Lai, Yi N. Lee, Yi J. Chen, Yao Chang Chen, Chih Chun Chen, Shih A. Chen, Cheng I. Lin, Cheng H. Tsai

研究成果: 雜誌貢獻文章

23 引文 斯高帕斯(Scopus)

摘要

We investigated the phenotypic features of cardiomyocytes, including the gap junctions, in the myocardial sleeve of thoracic veins. Single cardiomyocytes, isolated from the canine pulmonary veins (PV) and superior vena cava (SVC) using digestive enzymes, were examined by immunoconfocal microscopy using antisera against connexin43 (Cx43), Cx40, and other cell markers. The results showed that isolated cardiomyocytes displayed rod shapes of various sizes, ranging from 200 μm in length, and all the cells expressed α-actinin and vinculin. Gap junctions made of various amounts of Cx43 and Cx40 were found at the cell borders. These two connexins were extensively co-localized. Comparison between the thoracic veins showed that cells of the SVC contained more Cx43 gap junctions (total Cx43 gap junctions area per cell surface area, 4.0 ± 0.2% vs 1.5 ± 0.2%; p
原文英語
頁(從 - 到)259-266
頁數8
期刊Journal of Histochemistry and Cytochemistry
51
發行號2
出版狀態已發佈 - 二月 1 2003

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

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    Yeh, H. I., Lai, Y. J., Lee, Y. N., Chen, Y. J., Chen, Y. C., Chen, C. C., Chen, S. A., Lin, C. I., & Tsai, C. H. (2003). Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins. Journal of Histochemistry and Cytochemistry, 51(2), 259-266.