TY - JOUR
T1 - Differential effects of vegetable-derived indoles on the induction of quinone reductase in hepatoma cells
AU - Chen, Yue Hwa
AU - Yang, Darlene
PY - 2002/12
Y1 - 2002/12
N2 - The increased expression of quinone reductase (QR) has been associated with anticarcinogenic processes. The aim of this study was to explore the roles of the cruciferous vegetable-derived indoles, indole-3-carbinol (I3C) and indolo[3.2-b]carbazole (ICE), on the regulation of QR in both murine (Hepa-1) and human (HepG2) hepatoma cells. The results indicate that ICZ enhanced QR activity in both Hepa-1 and HepG2 cells, whereas its parent compound, I3C, had no significant effect on the induction of QR. Moreover, the ICZ-induced QR activity showed a higher response and expressed a more-significant dose-response in Hepa-1 cells. OR mRNA expression as analyzed by RT-PCR demonstrated a pattern similar to that of the enzyme activity. In conclusion, I3C did not show an enhancement effect on OR activity, but its acidic derivative, ICZ, increased the expression of QR mRNA, which then caused the augmentation of QR activity in Hepa-1 and HepG2 cells.
AB - The increased expression of quinone reductase (QR) has been associated with anticarcinogenic processes. The aim of this study was to explore the roles of the cruciferous vegetable-derived indoles, indole-3-carbinol (I3C) and indolo[3.2-b]carbazole (ICE), on the regulation of QR in both murine (Hepa-1) and human (HepG2) hepatoma cells. The results indicate that ICZ enhanced QR activity in both Hepa-1 and HepG2 cells, whereas its parent compound, I3C, had no significant effect on the induction of QR. Moreover, the ICZ-induced QR activity showed a higher response and expressed a more-significant dose-response in Hepa-1 cells. OR mRNA expression as analyzed by RT-PCR demonstrated a pattern similar to that of the enzyme activity. In conclusion, I3C did not show an enhancement effect on OR activity, but its acidic derivative, ICZ, increased the expression of QR mRNA, which then caused the augmentation of QR activity in Hepa-1 and HepG2 cells.
KW - Cruciferous vegetable
KW - Dietary indole
KW - Hepatoma cells
KW - Indolo[3,2-b]carbazole
KW - Quinone reductase
UR - http://www.scopus.com/inward/record.url?scp=0036971960&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036971960&partnerID=8YFLogxK
U2 - 10.3177/jnsv.48.477
DO - 10.3177/jnsv.48.477
M3 - Article
C2 - 12775114
AN - SCOPUS:0036971960
VL - 48
SP - 477
EP - 482
JO - The Journal of vitaminology
JF - The Journal of vitaminology
SN - 0301-4800
IS - 6
ER -