Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells

Pei Hsun Tsai, Chia Hsiung Cheng, Chun Yu Lin, Ying Tang Huang, Lung Ta Lee, Chithan C. Kandaswami, Yo Chuen Lin, Kevin Po Hao Lee, Chin Chun Hung, Jiuan Jiuan Hwang, Ferng Chun Ke, Geen Dong Chang, Ming Ting Lee

研究成果: 雜誌貢獻文章

20 引文 斯高帕斯(Scopus)

摘要

A highly invasive Du145-III subline was isolated by three successive passages of the parental Du145 prostate tumor cell line (Du145-P) through a Boyden chamber with matrigel-coated membrane support. Du145-III cells showed great invasion potential based on their increased ability to spread/migrate and enhanced expression/secretion of the matrix metalloproteinase 9 (MMP9). Du145-III cells exerted vasculogenic mimicry (VM) properties, reminiscent of endothelial cell characteristics and expressed elevated levels of cancer stem cell (CSC) markers, including Nanog, Sox2, CD44 and ABCG2 and ability to self-renew. Of prominence, MMP9 was required for the induction of VM and for increased stemness in Du145-III cells. Using Du145-III as a model, the effects of dietary flavonoids, luteolin and quercetin, were evaluated on stemness and invasion capacity of Du145-III cells in relation to JNK signaling pathway activation. These flavonoids depressed the malignancy of highly invasive Du145-III cells, VM, anchorage-independent spheroid formation and expression of certain CSC markers. Since luteolin and quercetin were able to target CSC cells and prevent cancer cell invasiveness, may serve as potential anti-angiogenesis and anti-metastasis agents.
原文英語
頁(從 - 到)6367-6380
頁數14
期刊Anticancer Research
36
發行號12
DOIs
出版狀態已發佈 - 十二月 1 2016

    指紋

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

引用此

Tsai, P. H., Cheng, C. H., Lin, C. Y., Huang, Y. T., Lee, L. T., Kandaswami, C. C., Lin, Y. C., Lee, K. P. H., Hung, C. C., Hwang, J. J., Ke, F. C., Chang, G. D., & Lee, M. T. (2016). Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells. Anticancer Research, 36(12), 6367-6380. https://doi.org/10.21873/anticanres.11234