Diagnosis of respiratory tract viruses in 24 h by immunofluorescent staining of shell vial cultures containing Madin-Darby Canine Kidney (MDCK) cells

Shin Ru Shih, Kuo Chien Tsao, Hsiao Chen Ning, Yhu Chering Huang, Tzou Yen Lin

研究成果: 雜誌貢獻文章

17 引文 (Scopus)

摘要

Nine hundred and seventy-eight clinical specimens were examined taken from patients with respiratory tract viruses (RV)-like syndrome between November 1996 and July 1998. The study was undertaken to evaluate the effectiveness of centrifuge-enhanced shell vial cultures (SVC) containing Madin-Darby Canine Kidney (MDCK) cells, combined with immunofluorescent (IF) staining in 24 h. This technique rapidly detects and identifies respiratory tract viruses. The conventional tube culture system with multiple cell lines would ordinarily detect RV within 3-30 days. The SVC/IF method using single cell line (MDCK cells) allowed detection of 81.5% of influenza A virus, 72% of parainfluenza virus, 82.6% of respiratory syncytial virus (RSV) and 79.6% of adenovirus in 24 h. Copyright (C) 1999 Elsevier Science B.V.
原文英語
頁(從 - 到)77-81
頁數5
期刊Journal of Virological Methods
81
發行號1-2
DOIs
出版狀態已發佈 - 八月 1999
對外發佈Yes

指紋

Madin Darby Canine Kidney Cells
Respiratory System
Staining and Labeling
Viruses
Paramyxoviridae Infections
Cell Line
Respiratory Syncytial Viruses
Influenza A virus
Adenoviridae

ASJC Scopus subject areas

  • Virology

引用此文

Diagnosis of respiratory tract viruses in 24 h by immunofluorescent staining of shell vial cultures containing Madin-Darby Canine Kidney (MDCK) cells. / Shih, Shin Ru; Tsao, Kuo Chien; Ning, Hsiao Chen; Huang, Yhu Chering; Lin, Tzou Yen.

於: Journal of Virological Methods, 卷 81, 編號 1-2, 08.1999, p. 77-81.

研究成果: 雜誌貢獻文章

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abstract = "Nine hundred and seventy-eight clinical specimens were examined taken from patients with respiratory tract viruses (RV)-like syndrome between November 1996 and July 1998. The study was undertaken to evaluate the effectiveness of centrifuge-enhanced shell vial cultures (SVC) containing Madin-Darby Canine Kidney (MDCK) cells, combined with immunofluorescent (IF) staining in 24 h. This technique rapidly detects and identifies respiratory tract viruses. The conventional tube culture system with multiple cell lines would ordinarily detect RV within 3-30 days. The SVC/IF method using single cell line (MDCK cells) allowed detection of 81.5{\%} of influenza A virus, 72{\%} of parainfluenza virus, 82.6{\%} of respiratory syncytial virus (RSV) and 79.6{\%} of adenovirus in 24 h. Copyright (C) 1999 Elsevier Science B.V.",
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AU - Lin, Tzou Yen

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