Development of bovine serum albumin-modified hybrid nanoclusters for magnetofluorescence imaging and drug delivery

Mochamad Zakki Fahmi, Keng Liang Ou, Jem Kun Chen, Ming Hua Ho, Shin Hwa Tzing, Jia Yaw Chang

研究成果: 雜誌貢獻文章

20 引文 斯高帕斯(Scopus)

摘要

In this study, bovine serum albumin (BSA) was used for simultaneously clustering and phase-transferring both oil-soluble AgInS2-ZnS quantum dots (QDs) and MnFe2O4 magnetic nanoparticles (MNPs) under ultrasonication. The hybrid nanoclusters, BSA(QMs), thus produced were conjugated with folic acid (FA) and doxorubicin (DOX) to improve their target specificity and drug delivery to HeLa cancer cells. The resulting nanoclusters were characterized by employing different analytical techniques, and the results showed the nanocluster magnetofluorescence property derived from the clustering process. It was also found that the hybrid nanoclusters were biocompatible, non-toxic, and considerably stable over a wide range of pH values and at high ionic strengths. In addition, the in vitro confocal microscopy and MR relaxation studies revealed the yellow fluorescence and T2 contrast-enhancing property of FA-BSA(QMs), as well as their cellular pathway to enter HeLa cells via folate receptor-mediated endocytosis. Furthermore, the cell viability data and flow cytometry results demonstrated the selective uptake of DOX-FA-BSA(QMs) by the HeLa cells, which significantly enhanced cell cytotoxicity. These results suggest that the proposed nanoclusters can be used as an effective and efficient strategy for magnetofluorescent probing and cancer drug delivery. This journal is

原文英語
頁(從 - 到)32762-32772
頁數11
期刊RSC Advances
4
發行號62
DOIs
出版狀態已發佈 - 2014

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

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  • 引用此

    Fahmi, M. Z., Ou, K. L., Chen, J. K., Ho, M. H., Tzing, S. H., & Chang, J. Y. (2014). Development of bovine serum albumin-modified hybrid nanoclusters for magnetofluorescence imaging and drug delivery. RSC Advances, 4(62), 32762-32772. https://doi.org/10.1039/c4ra05785f