Development of an information-rich LC-MS/MS database for the analysis of drugs in postmortem specimens

Hsiu Chuan Liu, Ray H. Liu, Hsiu O. Ho, Dong Liang Lin

研究成果: 雜誌貢獻文章同行評審

34 引文 斯高帕斯(Scopus)

摘要

With several instrument configurations available from various manufacturers, liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology is currently intensively studied for comprehensive drug screen and confirmation. An LC-MS/MS database, including 780 drug and toxic compounds, has been constructed, featuring information-rich MS/MS spectra derived from a novel fragmentation approach incorporating voltage ramping and broadened mass window for activation. The resulting spectra are rich in high- and low-mass fragment ions, highly effective for matching and proven reproducible over a 6 month test period. Coupled to effective sample preparation protocols, the database-searching process greatly improved the identification of drugs in postmortem specimens by the LC-electrospray ionization (ESI)-MS/MS technology. This method has significantly improved the efficiency of our routine laboratory operation that was based on a two-step [fluorescence polarization immunoassay (FPIA) and gas chromatography/mass spectrometry (GC/MS)] approach in the past.

原文英語
頁(從 - 到)9002-9011
頁數10
期刊Analytical Chemistry
81
發行號21
DOIs
出版狀態已發佈 - 11月 1 2009

ASJC Scopus subject areas

  • 分析化學

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