Development of an anti-methoxy poly(ethylene glycol) (α-mPEG) cell-based capture system to measure mPEG and mPEGylated molecules

Kuo Hsiang Chuang, Chien Han Kao, Steve R. Roffler, Ssu Jung Lu, Ta Chun Cheng, Yun Ming Wang, Chih Hung Chuang, Yuan Chin Hsieh, Yeng Tseng Wang, Jaw Yuan Wang, Kuo Yi Weng, Tian Lu Cheng

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)

摘要

Quantitative pharmacokinetic analysis of methoxy-poly(ethylene glycol) (mPEG) and mPEGylated molecules is important for clinical drug development. Here we developed sensitive sandwich and competitive ELISAs by expressing an anti-mPEG antibody on the surface of fibroblasts for effective capture of mPEG molecules in biological samples. α-mPEG sandwich ELISA could quantify the higher-molecular-weight of mPEG (2, 5, and 20 kDa) and mPEGylated molecules. α-mPEG cell-based competitive ELISA was developed to measure the lower-molecular-weight of mPEG molecules (559, 750, and 1000 Da) at nanomolar levels. In addition, α-mPEG cell-based ELISA was unaffected by the presence of 10% human serum or murine serum. We further demonstrate that the α-mPEG cell-based ELISA determined similar pharmacokinetics of mPEG5K as traditional gamma counting of 131I-mPEG5K. The α-mPEG cell-based ELISA may provide an accurate, high sensitivity and easy-to-use tool for directly measuring mPEG and mPEGylated molecules in complex biological samples to accelerate the clinical development of mPEG drugs.

原文英語
頁(從 - 到)6880-6888
頁數9
期刊Macromolecules
47
發行號19
DOIs
出版狀態已發佈 - 10月 14 2014

ASJC Scopus subject areas

  • 有機化學
  • 材料化學
  • 聚合物和塑料
  • 無機化學

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