Development of a selective three-dimensional HPLC system for enantiomer discriminated analysis of lactate and 3-hydroxybutyrate in human plasma and urine

Chin Ling Hsieh, Takeyuki Akita, Masashi Mita, Tomomi Ide, Jen Ai Lee, Kenji Hamase

研究成果: 雜誌貢獻文章同行評審

摘要

For the enantiomer discriminated determination of lactate (LA) and 3-hydroxybutyrate (3HB) in various complicated samples, a three-dimensional HPLC (3D-HPLC) system has been designed and developed by investigating the separation of the target analytes from unknown substances observed in the real target matrices. LA and 3HB were pre-column derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole for the sensitive fluorescence detection and introduced into the 3D-HPLC system composed of reversed-phase, mixed-mode and enantioselective separations. The present method was validated by calibration curves, precision and accuracy using standard solutions and human samples, and sufficient values were obtained. Using the method, the levels of D-LA, L-LA, D-3Hb and L-3HB were determined, and their concentrations were 9.9, 1004.2, 79.7 and 2.1 μM in the human plasma and 16.0, 86.6, 8.7 and 4.8 μM in the human urine, respectively. The present 3D-HPLC system could selectively determine trace amounts of the target hydroxy acid enantiomers without disturbance of the intrinsic interfering substances in complicated matrices and the applications to various disease samples are expected.
原文英語
文章編號113871
期刊Journal of Pharmaceutical and Biomedical Analysis
195
DOIs
出版狀態已發佈 - 二月 20 2021

ASJC Scopus subject areas

  • 分析化學
  • 藥學科學
  • 藥物發現
  • 光譜
  • 臨床生物化學

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