Development of a reversed-phase high-performance liquid Chromatographie method for analyzing furanocoμmarin components in citrus fruit juices and Chinese herbal medicines

Ying Ku Lin, Ming Thau Sheu, Chia Hui Huang, Hsiu O. Ho

研究成果: 雜誌貢獻文章同行評審

20 引文 斯高帕斯(Scopus)

摘要

A rapid and sensitive reversed-phase high performance liquid Chromatographie method for the quantitation of five furanocoumarins (bergaptol, psoralen, bergapten, 6',7'-dihydroxybergamottin, and bergamottin) is developed and validated. HPLC analysis of these five furanocoumarins is performed on a reversed-phase Inertsil ODS-2 column with a particle size of 5 μm Using only water and acetonitrile as solvents, good separation, good precision, and high accuracy are obtained for the analysis of furanocoμmarin components. This method is validated and applied to analyze the composition of five furanocoumarins in four citrus fruit juices (grapefruit, pomelo I, pomelo II, and shaddock) and ten Chinese herbal medicines (Bai-Zhi, Qiang-Huo, Du-Huo, Fang-Feng, Dang-Gui, Huang-Qin, Gan-Cao, Chen-Pi, Ge-Gen, and Yin-Chen-Hao) prepared by water decoction or an alcohol infusion. Results show that four of the five furanocoumarins (but not bergapten) are detected in grapefruit, pomelo I, and pomelo II, and the highest amount of these components is found in grapefruit juice. In the ten Chinese herbal medicines, the five furanocoumarins are not detected in Ge-Gen or Yin-Chen-Hao. The remaining herbs contain various compositions and amounts of furanocoμmarins. In general, Chinese herbal medicines prepared by the 40% ethanol infusion contain larger amounts of furanocoumarins than those prepared by hot water decoction.

原文英語
頁(從 - 到)211-215
頁數5
期刊Journal of Chromatographic Science
47
發行號3
出版狀態已發佈 - 三月 2009

ASJC Scopus subject areas

  • 分析化學

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