Developement of valve-integrated nanofluidic preconcentrator for low-abundance protein detection

Chih Zong Deng, Yu Jui Fan, Horn Jiunn Sheen

研究成果: 書貢獻/報告類型會議貢獻

摘要

In this study, we developed a new immunosensor that can achieve ultra-low detection limit and high sensitivity. This new device has an electrokinetic trapping (EKT)-based nanofluidic preconcentrator, which was integrated with oscillating bubble valves, to trap concentrated antigen and immunobeads. During the immunoassay process, oscillating bubbles rapidly grew and acted as control valves and to block the microchannel. Thereafter, the trapped preconcentrated antigen plug and antibody-coated nanobeads were preserved in the region between these two valves. Finally, the antigen concentration was quantitatively analyzed by a real-time measurement of Brownian diffusion of the immuno-beads. In this work, the test sample used was C-reactive protein (CRP) which is a risk indicator of coronary heart dis-ease and atherosclerosis.
原文英語
主出版物標題22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
發行者Chemical and Biological Microsystems Society
頁面853-856
頁數4
ISBN(電子)9781510897571
出版狀態已發佈 - 一月 1 2018
事件22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, 臺灣
持續時間: 十一月 11 2018十一月 15 2018

出版系列

名字22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
2

會議

會議22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
國家/地區臺灣
城市Kaohsiung
期間11/11/1811/15/18

ASJC Scopus subject areas

  • 化學 (全部)
  • 生物工程
  • 化學工程(雜項)
  • 控制與系統工程

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