Determination of sesamin and sesamolin by using HPLC with an internal standard

Wei Ju Lee, Poi Yik Teh, Nan Wei Su, Min Hsiung Lee

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

Major lignans in sesame seeds are sesamin and sesamolin. Numerous studies have reported that these lignans have physiological functions. However, there are few researches regarding the methods for quantification of sesamin and sesamolin. Thus, the results varied significantly due to the different quantitative methods used in reported studies. In the present study, we used naphthalene as an internal standard to establish an HPLC method to quantify the contents of sesamin and sesamolin. The results of validation experiments indicated that this quantitative method developed in this study could provide a reasonable method for determination of sesamin and sesamolin with high recoveries, accuracy and precision. It takes 25 minutes to finish one analysis. The LOQ of sesamin is 75 μg/L, and the LOQ of sesamolin is 150 μg/L. We also used this method to determine the sesamin and sesamolin contents of the commercial products, and compared with their labeled amounts. This method could be a reference for the industry and authorities to regulate the lignan contents in sesame-related commercial products.
原文英語
頁(從 - 到)102-108
頁數7
期刊Taiwanese Journal of Agricultural Chemistry and Food Science
51
發行號2
出版狀態已發佈 - 1月 1 2013
對外發佈

ASJC Scopus subject areas

  • 食品科學
  • 應用微生物與生物技術
  • 農學與作物科學

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