Determination of protein-unbound rhynchiphylline brain distribution by microdialysis and ultra-performance liquid chromatography with tandem mass spectrometry

Chia Jung Lee, Thomas Y. Hsueh, Lie Chwen Lin, Tung Hu Tsai

研究成果: 雜誌貢獻文章同行評審

10 引文 斯高帕斯(Scopus)

摘要

The stem with hook of Uncaria rhynchophylla (Chinese herbal name Gou-Teng) is a traditional Chinese medicine that has been ethnopharmacologically used to extinguish wind and clean interior heat. Rhynchophylline (RHY), a tetracyclic oxindole alkaloid isolated from U. rhynchophylla, displays significant antineuroinflammatory effects. However, there is no evidence to indicate that rhynchophylline can cross the blood-brain barrier and be detected in the brain. In this study, an in vivo microdialysis sampling method coupled with UPLC/MS/MS was employed for the continuous simultaneous monitoring of unbound RHY in rat blood and brain. The precursor ion→product ion transition at m/z 385.2→160.0 for rhynchophylline was monitored. A calibration curve gave good linearity (r>0.996) over the concentration range from 0.5 to 1000ng/mL. The results demonstrated that rhynchophylline could be detected in the brain and plasma from 15min to 6h after its administration (1 or 10mg/kg, i.v.). All the pharmacokinetic parameters of rhynchophylline in the brain and plasma were obtained. These results show that rhynchophylline can cross the blood-brain barrier and they provide useful clinical information.

原文英語
頁(從 - 到)901-906
頁數6
期刊Biomedical Chromatography
28
發行號6
DOIs
出版狀態已發佈 - 2014
對外發佈Yes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Pharmacology
  • Medicine(all)

指紋 深入研究「Determination of protein-unbound rhynchiphylline brain distribution by microdialysis and ultra-performance liquid chromatography with tandem mass spectrometry」主題。共同形成了獨特的指紋。

引用此