Determination of isoniazid methanesulphonate and its metabolites in rabbit blood by high-performance liquid chromatography

Kuang Yang Hsu, Yih Ho

研究成果: 雜誌貢獻文章同行評審

9 引文 斯高帕斯(Scopus)

摘要

A rapid and sensitive high-performance liquid chromatographic method is described for simultaneous determination of isoniazid methanesulphonate (IHMS) and its metabolites, such as isoniazid (INH) and acetylisoniazid (AcINH) in rabbit blood. According to stability studies, IHMS was most stable at pH 3-5. After acidifying the blood to pH 5.0, a suitable amount of acetonitrile was added to the supernatant for extraction and niacinamide served as an internal standard. After evaporation, the residue was reconstituted with phosphate buffer and aliquots of this solution were separated on a reversed-phase phenyl column by a mobile phase consisting of 0.25 mM tetrabutylammonium phosphate as a paired-ion reagent. UV detection was performed at 280 nm. Under these conditions, the between-run coefficients of variation of IHMS, INH and AcINH from 1 to 25 μm/ml were 4.7 ± 2.5, 5.4 ± 1.0 and 5.1 ± 3.1%, respectively. Hence this sensitive, reproducible and accurate method was suitable for pharmacokinetic studies of IHMS.

原文英語
頁(從 - 到)305-312
頁數8
期刊Journal of Chromatography B: Biomedical Sciences and Applications
493
發行號C
DOIs
出版狀態已發佈 - 1989

ASJC Scopus subject areas

  • 化學 (全部)
  • 臨床生物化學
  • 分子醫學

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