Determination of (+)-catechin in plasma by high-performance liquid chromatography using fluorescence detection

Yih Ho, Yueh Lin Lee, Kuang Yang Hsu

研究成果: 雜誌貢獻文章

59 引文 (Scopus)

摘要

A high-performance liquid chromatographic method, using fluorescence detection, was developed for the determination of (+)-catechin in rabbit plasma. The procedure involved the precipitation of plasma protein using acetonitrile, followed by solid-phase adsorption onto alumina. After washing with water and methanol, the residue was vortex-mixed with perchloric acid solution to release the adsorbed (+)-catechin. Separation was performed on a reversed-phase column using an eluent consisting of phosphoric acid solution with 12% acetonitrile. The excitation and emission wavelengths were set at 280 and 310 nm, respectively. The retention times for (+)-catechin and the internal standard (deoxyhigenamine) were 6.87 and 8.47 min respectively, without any interference. Validations of accuracy and precision were satisfactory in both within- and between-run assays. All coefficients of variance were less than 6% and mean relative errors were within ± 3.75%. The average recovery was 73.77%. The limit of detection and quantitation were 1 ng and 0.02 μg/ml, respectively. Application of this method was successfully assessed by intravenous administration of a 15 mg/kg dose of (+)-catechin in rabbits. This new method provides a simple, specific and sensitive determination for (+)-catechin in rabbit plasma and is suitable for pharmacokinetic studies.

原文英語
頁(從 - 到)383-389
頁數7
期刊Journal of Chromatography B: Biomedical Sciences and Applications
665
發行號2
DOIs
出版狀態已發佈 - 三月 24 1995

指紋

Catechin
High performance liquid chromatography
Fluorescence
Plasmas
Pharmacokinetics
Aluminum Oxide
Washing
Methanol
Blood Proteins
Assays
Vortex flow
Adsorption
Recovery
Wavelength
Water
Liquids
acetonitrile

ASJC Scopus subject areas

  • Chemistry(all)

引用此文

Determination of (+)-catechin in plasma by high-performance liquid chromatography using fluorescence detection. / Ho, Yih; Lee, Yueh Lin; Hsu, Kuang Yang.

於: Journal of Chromatography B: Biomedical Sciences and Applications, 卷 665, 編號 2, 24.03.1995, p. 383-389.

研究成果: 雜誌貢獻文章

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abstract = "A high-performance liquid chromatographic method, using fluorescence detection, was developed for the determination of (+)-catechin in rabbit plasma. The procedure involved the precipitation of plasma protein using acetonitrile, followed by solid-phase adsorption onto alumina. After washing with water and methanol, the residue was vortex-mixed with perchloric acid solution to release the adsorbed (+)-catechin. Separation was performed on a reversed-phase column using an eluent consisting of phosphoric acid solution with 12{\%} acetonitrile. The excitation and emission wavelengths were set at 280 and 310 nm, respectively. The retention times for (+)-catechin and the internal standard (deoxyhigenamine) were 6.87 and 8.47 min respectively, without any interference. Validations of accuracy and precision were satisfactory in both within- and between-run assays. All coefficients of variance were less than 6{\%} and mean relative errors were within ± 3.75{\%}. The average recovery was 73.77{\%}. The limit of detection and quantitation were 1 ng and 0.02 μg/ml, respectively. Application of this method was successfully assessed by intravenous administration of a 15 mg/kg dose of (+)-catechin in rabbits. This new method provides a simple, specific and sensitive determination for (+)-catechin in rabbit plasma and is suitable for pharmacokinetic studies.",
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