Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis

Wei Kung Wang, Shey Ying Chen, I. Jung Liu, Yee Chun Chen, Hui Ling Chen, Chao Fu Yang, Pei Jer Chen, Shiou Hwei Yeh, Chuan Liang Kao, Li Min Huang, Po Ren Hsueh, Jann Tay Wang, Wang Hwei Sheng, Chi Tai Fang, Chien Ching Hung, Szu Min Hsieh, Chan Ping Su, Wen Chu Chiang, Jyh Yuan Yang, Jih Hui LinSzu Chia Hsieh, Hsien Ping Hu, Yu Ping Chiang, Jin Town Wang, Pan Chyr Yang, Shan Chwen Chang

研究成果: 雜誌貢獻文章同行評審

136 引文 斯高帕斯(Scopus)

摘要

The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little is known about the load of SARS-CoV in oral droplets. We examined oral specimens, including throat wash and saliva, and found large amounts of SARS-CoV RNA in both throat wash (9.58 × 102 to 5.93 × 106 copies/mL) and saliva (7.08 × 103 to 6.38 × 10 8 copies/mL) from all specimens of 17 consecutive probable SARS case-patients, supporting the possibility of transmission through oral droplets, Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstrating the possibility of developing a convenient antigen detection assay. This finding, with the high detection rate a median of 4 days after disease onset and before the development of lung lesions in four patients, suggests that throat wash and saliva should be included in sample collection guidelines for SARS diagnosis.

原文英語
頁(從 - 到)1213-1219
頁數7
期刊Emerging Infectious Diseases
10
發行號7
DOIs
出版狀態已發佈 - 一月 1 2004
對外發佈

ASJC Scopus subject areas

  • 流行病學
  • 微生物學(醫學)
  • 傳染性疾病

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