Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers

Nan Yung Hsu, Chih Yi Chen, Chii Huei Wu, Tse Fia Liu, Po Cheung Kwam, Chung Ping Hsu, Jiun Yi Hsia, Wu Tsung Chang

研究成果: 雜誌貢獻文章

3 引文 斯高帕斯(Scopus)

摘要

Point mutations of the K-ras gene have been reported in a wide variety of human tumors. By using polymerase chain reaction followed by direct DNA sequencing, we screened for point mutations at codons 12 and 13 of the K-ras gene in specimens obtained from fresh frozen tumors in 38 patients with non-small cell lung cancers. Point mutations were detected in two of 38 (5.3%) resected non-small cell lung cancers. Both of them were G to T transversions. One patient was found to have a K-ras codon 13 point mutation (GGC to TGC, gly to cys), while the other had a codon 12 point mutation (GGT to GTT, gly to val). Based on the limited numbers in this study, we found that the frequency of K-ras point mutations in codons 12 and 13 among Asian patients with lung adenocarcinomas was lower than that detected among Caucasian patients.
原文英語
頁(從 - 到)741-745
頁數5
期刊Journal of the Formosan Medical Association = Taiwan yi zhi
95
發行號10
出版狀態已發佈 - 1996
對外發佈Yes

ASJC Scopus subject areas

  • Medicine(all)

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  • 引用此

    Hsu, N. Y., Chen, C. Y., Wu, C. H., Liu, T. F., Kwam, P. C., Hsu, C. P., Hsia, J. Y., & Chang, W. T. (1996). Detection of K-ras point mutations in codons 12 and 13 in non-small cell lung cancers. Journal of the Formosan Medical Association = Taiwan yi zhi, 95(10), 741-745.