Detection of epstein-barr virus genome in nasopharyngeal carcinoma by in situ DNA hybridization

Chi Long Chen, Wu Nan Wen, Jen Yang Chen, Mow Ming Hsu, Hey Chi Hsu

研究成果: 雜誌貢獻文章

55 引文 (Scopus)

摘要

To elucidate the possible role of Epstein-Barr virus (EBV) in nasopharyngeal carcinoma (NPC), we investigated the EBV genome in NPC by in situ DNA hybridization using radioisotope- and biotin-labeled EBV DNA probes. The EBV genome was detected in the tumor cells in all (100%) 60 cases, irrespective of histological type, but not in the lymphocytes. Silver grains, which reflected the copy number of the EBV genome, were more abundant in the nonkeratinizing, spindle cell, and undifferentiated carcinomas than in keratinizing squamous cell carcinoma. In the keratinizing carcinoma, which was poorly differentiated in this series, the EBV genome was usually detected in anaplastic tumor cells, and not in the keratinizing areas. The sensitivity of the radioisotopic technique was superior to that of the biotinylated probe method (100 vs. 81.7%, p<0.0003). These results suggest that EBV is etiologically related to nasopharyngeal carcinogenesis and that the differentiation of tumor cells in vivo, and probably also in vitro, may become incompatible with EBV replication.

原文英語
頁(從 - 到)91-98
頁數8
期刊Intervirology
36
發行號2
DOIs
出版狀態已發佈 - 1993
對外發佈Yes

指紋

Carcinoma in Situ
Human Herpesvirus 4
In Situ Hybridization
Genome
DNA
Carcinoma
Neoplasms
Nasopharyngeal carcinoma
DNA Probes
Virus Replication
Biotin
Silver
Radioisotopes
Cell Differentiation
Squamous Cell Carcinoma
Carcinogenesis
Lymphocytes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

引用此文

Detection of epstein-barr virus genome in nasopharyngeal carcinoma by in situ DNA hybridization. / Chen, Chi Long; Wen, Wu Nan; Chen, Jen Yang; Hsu, Mow Ming; Hsu, Hey Chi.

於: Intervirology, 卷 36, 編號 2, 1993, p. 91-98.

研究成果: 雜誌貢獻文章

Chen, Chi Long ; Wen, Wu Nan ; Chen, Jen Yang ; Hsu, Mow Ming ; Hsu, Hey Chi. / Detection of epstein-barr virus genome in nasopharyngeal carcinoma by in situ DNA hybridization. 於: Intervirology. 1993 ; 卷 36, 編號 2. 頁 91-98.
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