Detection of cefotaxime-resistant CTX-M-3 in clinical isolates of Serratia marcescens

Mei Fen Tsou, Wen Liang Yu, Wan Hung Siu, Chizh Ju Chi, [No Value] Chieh-Ju, Ching Chen Ssu, Lii Tzu Wu

研究成果: 雜誌貢獻文章同行評審

3 引文 斯高帕斯(Scopus)


Strains of Serratia marcescens (isolated in a hospital during April and August 2000) resistant to ampicillin, chloramphenicol, trimethoprim- sulfamethoxazole, streptomycin, tetracycline, and gentamicin were characterized. Out of a total of 34 clinical isolates 6 (17.6 %) exhibited the extended spectrum β-lactamases (ESBL) resistance; they were also resistant to cefotaxime (minimum inhibitory concentration, MIC ≥ 128 μg/mL) but susceptible to imipenem (MIC ≤ 0.5 μg/mL). This multidrug resistance was shown to be transferred by a conjugative plasmid. Transconjugants revealed similar MIC profiles when compared to the parental strains. Isoelectric focusing revealed one major transferable β-lactamase (pI 8.4) which was further identified as CTX-M-3 by PCR and gene sequencing. The presence of strains with this type of ESBL showed the evolution of bla genes and their dissemination among at least three species of the family Enterobacteriaceae isolated within a single hospital. The predominance of CTX-M type enzymes found in this area of Taiwan appeared to be similar to that described in Poland.

頁(從 - 到)452-456
期刊Folia Microbiologica
出版狀態已發佈 - 2004

ASJC Scopus subject areas

  • 微生物學


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