Detection of C-reactive protein in evanescent wave field usingmicroparticle-tracking velocimetry

Yu Jui Fan, Horn Jiunn Sheen, Yi Hsing Liu, Jing Fa Tsai, Tzu Heng Wu, Kuang Chong Wu, Shiming Lin

研究成果: 雜誌貢獻文章同行評審

18 引文 斯高帕斯(Scopus)

摘要

A new technique is developed to measure the nanoparticles' Brownian motions by employing microparticle-tracking velocimetry (micro-PTV) in evanescent wave field, which can provide high signal-to-noise ratio images for analyzing nanoparticles' movements. This method enables real-time detection of C-reactive proteins (CRPs) during the rapid interaction between CRPs and anti-CRP-coated nanobeads as CRP concentrations are related to the nanobeads' Brownian velocity in the equilibrium state. The smallest observable nanobeads with 185 nm were utilized in this experiment to detect CRP concentrations as low as 0.1 μg/mL even in a high-viscosity solution. Further, the dissociation constant, KD, can be evaluated based on the experimental results.
原文英語
頁(從 - 到)13751-13754
頁數4
期刊Langmuir
26
發行號17
DOIs
出版狀態已發佈 - 九月 7 2010
對外發佈

ASJC Scopus subject areas

  • 電化學
  • 凝聚態物理學
  • 表面和介面
  • 材料科學(全部)
  • 光譜

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