Detecting Mycobacterium Tuberculosis in BACTEC MGIT 960 Cultures by COBAS AMPLICOR MTB in Routine Clinical Practice

白 冠壬(Kuan-Jen Bai), 余 芳蘭(Fang-Lan Yu), 周 如文(Ru-Wen Jou), Mei Shiang Wang, 林 明輝(Ming-Hwei Lin), 李 俊年(Chun-Nin Lee), 余 明治(Ming-Chih Yu)

研究成果: 雜誌貢獻文章

摘要

前言:快速、自動的結核菌培養偵測系統BACTEC MGIT 960已在台灣廣為應用;然而,目前非結核性分枝桿菌在臨床實驗室的高分離率也造成診斷上的困擾。此研究主要目的為評估COBAS AMPLICOR MTB在BACTEC MGIT 960陽性檢體的臨床應用。
研究方法:從2006年3月1日到2007年2月28日共有270個BACTEC MGIT 960陽性檢体在台北醫學大學•萬芳醫院進行COBAS AMPLICOR MTB檢驗,並與傳統生化菌株鑑定的結果進行比較。
結果:207(76.7%)個COBAS AMPLICOR MTB檢驗有決定性結論,63(3株為結核菌,60株為非結核性分枝桿菌)個檢驗無決定性結論。在176個結果為陽性者,174個檢驗為真陽性,2個檢驗為偽陽性。在31個結果為陰性者,30個檢驗為真陰性,1個檢驗為偽陰性。若不考慮檢驗結果為無決定性結論者,此項應用的敏感性、特異性、陽性預測值與陰性預測值分別為99%、94%、99%及97%。
結論:COBAS AMPLICOR MTB可能適合作為BACTEC MGIT 960陽性檢體快速偵測及鑑定結核菌的檢驗工具。
原文英語
頁(從 - 到)156-163
頁數8
期刊胸腔醫學
23
發行號3
出版狀態已發佈 - 2008

指紋

Mycobacterium tuberculosis
Nontuberculous Mycobacteria
Taiwan
Sensitivity and Specificity

Keywords

  • 結核菌
  • COBAS AMPLICOR MTB
  • BACTEC MGIT 960
  • Mycobacterium tuberculosis
  • BACTEC MGIT 960

引用此文

Detecting Mycobacterium Tuberculosis in BACTEC MGIT 960 Cultures by COBAS AMPLICOR MTB in Routine Clinical Practice. / 白冠壬(Kuan-Jen Bai); 余芳蘭(Fang-Lan Yu); 周如文(Ru-Wen Jou); Wang, Mei Shiang; 林明輝(Ming-Hwei Lin); 李俊年(Chun-Nin Lee); 余明治(Ming-Chih Yu).

於: 胸腔醫學, 卷 23, 編號 3, 2008, p. 156-163.

研究成果: 雜誌貢獻文章

白冠壬(Kuan-Jen Bai) ; 余芳蘭(Fang-Lan Yu) ; 周如文(Ru-Wen Jou) ; Wang, Mei Shiang ; 林明輝(Ming-Hwei Lin) ; 李俊年(Chun-Nin Lee) ; 余明治(Ming-Chih Yu). / Detecting Mycobacterium Tuberculosis in BACTEC MGIT 960 Cultures by COBAS AMPLICOR MTB in Routine Clinical Practice. 於: 胸腔醫學. 2008 ; 卷 23, 編號 3. 頁 156-163.
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title = "Detecting Mycobacterium Tuberculosis in BACTEC MGIT 960 Cultures by COBAS AMPLICOR MTB in Routine Clinical Practice",
abstract = "Introduction: The rapid, automated cultivation and detection system, BACTEC MGIT 960, is widely used in Taiwan. But the high nontuberculous mycobacteria (NTM) isolation rate is a concern that should be carefully evaluated. The aim of this study was to evaluate the ability of the commercial COBAS AMPLICOR MTB amplification system to identify Mycobacterium tuberculosis (M. tuberculosis) in positive BACTEC MGIT 960 cultures in routine clinical practice.Methods: We tested 270 positive BACTEC MGIT 960 cultures with the COBAS AMPLICOR MTB at Taipei Medical University-Wan Fang Hospital from March 1, 2006 through February 28, 2007. The COBAS AMPLICOR MTB results were compared with mycobacterial species identification by conventional biochemical testing.Results: We found that 207 (76.7{\%}) COBAS AMPLICOR MTB results were regarded as conclusive, and 63 (3 M. tuberculosis, 60 NTM) inconclusive. Among 176 conclusive results positive for M. tuberculosis, 174 were regarded as true positive and 2 false positive. Among 31 conclusive results negative for M. tuberculosis, 30 were regarded as true negative and 1 false negative. After excluding the inconclusive results, we further found that the sensitivity, specificity, and positive/negative predictive values of the COBAS AMPLICOR MTB test were 99, 94, 99, and 97{\%}, respectively.Conclusion: COBAS AMPLICOR MTB might be suitable for rapid detection and identification of M. tuberculosis in BACTEC MGIT 960 cultures in routine clinical practice.",
keywords = "結核菌, COBAS AMPLICOR MTB, BACTEC MGIT 960, Mycobacterium tuberculosis, COBAS AMPLICOR MTB, 結核菌, COBAS AMPLICOR MTB, BACTEC MGIT 960, Mycobacterium tuberculosis , BACTEC MGIT 960",
author = "白, {冠壬(Kuan-Jen Bai)} and 余, {芳蘭(Fang-Lan Yu)} and 周, {如文(Ru-Wen Jou)} and Wang, {Mei Shiang} and 林, {明輝(Ming-Hwei Lin)} and 李, {俊年(Chun-Nin Lee)} and 余, {明治(Ming-Chih Yu)}",
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T1 - Detecting Mycobacterium Tuberculosis in BACTEC MGIT 960 Cultures by COBAS AMPLICOR MTB in Routine Clinical Practice

AU - 白, 冠壬(Kuan-Jen Bai)

AU - 余, 芳蘭(Fang-Lan Yu)

AU - 周, 如文(Ru-Wen Jou)

AU - Wang, Mei Shiang

AU - 林, 明輝(Ming-Hwei Lin)

AU - 李, 俊年(Chun-Nin Lee)

AU - 余, 明治(Ming-Chih Yu)

PY - 2008

Y1 - 2008

N2 - Introduction: The rapid, automated cultivation and detection system, BACTEC MGIT 960, is widely used in Taiwan. But the high nontuberculous mycobacteria (NTM) isolation rate is a concern that should be carefully evaluated. The aim of this study was to evaluate the ability of the commercial COBAS AMPLICOR MTB amplification system to identify Mycobacterium tuberculosis (M. tuberculosis) in positive BACTEC MGIT 960 cultures in routine clinical practice.Methods: We tested 270 positive BACTEC MGIT 960 cultures with the COBAS AMPLICOR MTB at Taipei Medical University-Wan Fang Hospital from March 1, 2006 through February 28, 2007. The COBAS AMPLICOR MTB results were compared with mycobacterial species identification by conventional biochemical testing.Results: We found that 207 (76.7%) COBAS AMPLICOR MTB results were regarded as conclusive, and 63 (3 M. tuberculosis, 60 NTM) inconclusive. Among 176 conclusive results positive for M. tuberculosis, 174 were regarded as true positive and 2 false positive. Among 31 conclusive results negative for M. tuberculosis, 30 were regarded as true negative and 1 false negative. After excluding the inconclusive results, we further found that the sensitivity, specificity, and positive/negative predictive values of the COBAS AMPLICOR MTB test were 99, 94, 99, and 97%, respectively.Conclusion: COBAS AMPLICOR MTB might be suitable for rapid detection and identification of M. tuberculosis in BACTEC MGIT 960 cultures in routine clinical practice.

AB - Introduction: The rapid, automated cultivation and detection system, BACTEC MGIT 960, is widely used in Taiwan. But the high nontuberculous mycobacteria (NTM) isolation rate is a concern that should be carefully evaluated. The aim of this study was to evaluate the ability of the commercial COBAS AMPLICOR MTB amplification system to identify Mycobacterium tuberculosis (M. tuberculosis) in positive BACTEC MGIT 960 cultures in routine clinical practice.Methods: We tested 270 positive BACTEC MGIT 960 cultures with the COBAS AMPLICOR MTB at Taipei Medical University-Wan Fang Hospital from March 1, 2006 through February 28, 2007. The COBAS AMPLICOR MTB results were compared with mycobacterial species identification by conventional biochemical testing.Results: We found that 207 (76.7%) COBAS AMPLICOR MTB results were regarded as conclusive, and 63 (3 M. tuberculosis, 60 NTM) inconclusive. Among 176 conclusive results positive for M. tuberculosis, 174 were regarded as true positive and 2 false positive. Among 31 conclusive results negative for M. tuberculosis, 30 were regarded as true negative and 1 false negative. After excluding the inconclusive results, we further found that the sensitivity, specificity, and positive/negative predictive values of the COBAS AMPLICOR MTB test were 99, 94, 99, and 97%, respectively.Conclusion: COBAS AMPLICOR MTB might be suitable for rapid detection and identification of M. tuberculosis in BACTEC MGIT 960 cultures in routine clinical practice.

KW - 結核菌

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KW - COBAS AMPLICOR MTB

KW - 結核菌

KW - COBAS AMPLICOR MTB

KW - BACTEC MGIT 960

KW - Mycobacterium tuberculosis

KW - BACTEC MGIT 960

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