Desipramine activated Bcl-2 expression and inhibited lipopolysaccharide- induced apoptosis in hippocampus-derived adult neural stem cells

Yu Yin Huang, Chi Hsien Peng, Yi Ping Yang, Chih Chiau Wu, Wen-Ming Hsu, Hsiao Jung Wang, Kwok Han Chan, Yi Pen Chou, Shih Jen Chen, Yuh Lih Chang

研究成果: 雜誌貢獻文章同行評審

56 引文 斯高帕斯(Scopus)

摘要

Desipramine (DP) is a tricyclic antidepressant used for treating depression and numerous other psychiatric disorders. Recent studies have shown that DP can promote neurogenesis and improve the survival rate of hippocampal neurons. However, whether DP induces neuroprotection or promotes the differentiation of neural stem cells (NSCs) needs to be elucidated. In this study, we cultured NSCs derived from the hippocampal tissues of adult rats as an in vitro model to evaluate the modulation effect of DP on NSCs. First, we demonstrated that the expression of Bcl-2 mRNA and nestin in 2 μM DP-treated NSCs were up-regulated and detected by real-time reverse transcriptase polymerase chain reaction (RT-PCR). The results of Western blotting and immunofluorescent study confirmed that Bcl-2 protein expression was significantly increased in Day 3 DP-treated NSCs. Using the Bcl-2 small interfering RNA (siRNA) method, our results further showed that DP protects the lipopolysaccharide (LPS)-induced apoptosis in NSCs, in part by activating the expression of Bcl-2. Furthermore, DP treatment significantly inhibited the induction of proinflammatory factor interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in the culture medium of LPS-treated NSCs mediated by Bcl-2 modulation. The results of high performance liquid chromatography coupled to electro-chemical detection further confirmed that DP significantly increased the functional production of serotonin (26 ± 3.5 μM, DP-treated 96 h) and noradrenaline (50 ± 8.9 μM, DP-treated 96 h) in NSCs through activation of the MAPK/ERK pathway and partially mediated by Bcl-2. In conclusion, the present results indicate that DP can increase neuroprotection ability by inhibiting the LPS-induced inflammatory process in NSCs via the modulation of Bcl-2 expression, as confirmed by the siRNA method.

原文英語
頁(從 - 到)61-72
頁數12
期刊Journal of Pharmacological Sciences
104
發行號1
DOIs
出版狀態已發佈 - 五月 28 2007
對外發佈Yes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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