Dendritic cells modulated by cytokine-expressing adenoviruses alleviate eosinophilia and airway hyperresponsiveness in an animal model of asthma

Yi Ling Ye, Yueh Lun Lee, Zen Jai Chuang, Huai Jean Lai, Chun Chi Chen, Mi Hua Tao, Bor Luen Chiang

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10 引文 斯高帕斯(Scopus)

摘要

Background It has been found that T H1-related cytokines can decrease the accumulation of eosinophils in lung tissue and relieve airway constriction. Objective Dendritic cells (DCs) have been found to prime naive T-helper cells efficiently. In this study, DCs infected with T H1 cytokine-expressing adenovirus can be used to induce antigen-specific T H1 cells for treatment in an animal model of asthma. Methods Cytokine gene-modulated DCs pulsed with ovalbumin antigen (OVA) were injected intravenously into naive mice 1 week before sensitization with OVA antigen. The mice were then monitored for OVA-specific IgE, airway inflammatory cell infiltration, and airway hyperresponsiveness in the study. Results Significant levels of IL-12 or IL-18 were expressed by Ad-IL-12 or Ad-IL-18 infected, bone marrow-derived DCs. Ad-IL-12 and Ad-IL-18 co-infected DCs effectively, decreasing sera anti-OVA IgE antibody levels, lung eosinophilia, and airway hyperresponsiveness. Conclusion We concluded that DCs modulated by T H1-prone cytokine-expressing adenoviruses can alleviate T H2-type airway inflammation in a murine model and can provide possible therapeutic application for DCs in asthma.

原文英語
頁(從 - 到)88-96
頁數9
期刊Journal of Allergy and Clinical Immunology
114
發行號1
DOIs
出版狀態已發佈 - 七月 2004
對外發佈Yes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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