Dehydroascorbate reductase and monodehydroascorbate reductase activities of trypsin inhibitors, the major sweet potato (Ipomoea batatas [L.] Lam) root storage protein

Wen Chi Hou, Yaw Huei Lin

研究成果: 雜誌貢獻文章同行評審

56 引文 斯高帕斯(Scopus)

摘要

Trypsin inhibitors (TIs) were purified from the storage roots of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57) with ammonium sulfate precipitation, Sephadex G-75 gel filtration chromatography, and trypsin- Sepharose 4B affinity chromatography as previously reported [1]. Dehydroascorbate (DHA) was reduced by TIs independent of glutathione to regenerate ascorbate (AsA) contrasting with the function of DHA reductase with glutathione as a cofactor. Intermolecular thiol-disulfide interchanges of TIs were found during DHA reduction. AsA was oxidized by AsA oxidase to generate monodehydroascorbate (MDA) free radicals. MDA was reduced also by TIs to AsA in the presence of NADH which was the function of MDA reductase. The physiological significance of TIs with both DHA reductase and MDA reductase activities are discussed.

原文英語
頁(從 - 到)151-158
頁數8
期刊Plant Science
128
發行號2
DOIs
出版狀態已發佈 - 十月 13 1997
對外發佈

ASJC Scopus subject areas

  • 遺傳學
  • 農學與作物科學
  • 植物科學

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