Defective functions of circulating CD4+CD25+ and CD4+CD25- T cells in patients with chronic ordinary urticaria

Wu Charng Chen, Bor Luen Chiang, H. Eugene Liu, Sy Jye Leu, Yueh Lun Lee

研究成果: 雜誌貢獻文章

17 引文 (Scopus)

摘要

Background: Patients with chronic ordinary urticaria (CU) are divided into two groups: 30-50% have chronic autoimmune urticaria, and the remainder have chronic idiopathic urticaria. CD4+CD25+ regulatory T (Treg) cells play critical roles in maintaining peripheral tolerance and preventing autoimmunity, but the characteristics of Treg cells have not yet been defined in CU. Objective: To identify whether CD4+ T cells play an important immunoregulatory role in the etiology of CU, we determined the frequencies and functions of circulating CD4+CD25+ and CD4+CD25- T cells in CU patients and healthy control subjects, with special focus on the characteristics of CD4+CD25+ T cells. Methods: Peripheral blood mononuclear cells (PBMCs) were obtained from CU and healthy controls in this study. The frequency of CD4+CD25+ T cells in PBMCs was detected by flow cytometry. The expression levels of forkhead box P3 (FOXP3) and transforming growth factor-β (TGF-β) in CD4+CD25+ T cells were detected by real-time PCR. Furthermore, the suppressive function of CD4+CD25+ T cells was analyzed. Additionally, the Th1/Th2 cytokine secretory profile in mitogen-stimulated CD4+CD25- T cells was measured by ELISA. Results: An increased frequency of CD4+CD25+ T cells was observed in CU patients (n = 19) compared to control subjects (n = 7). No significant difference was detected in the expression levels of FOXP3 or TGF-β between CU patients (n = 14) and control subjects (n = 7). Strikingly, the suppressive capacity of CD4+CD25+ Treg cells from 2 of 5 CU patients was partially defective. We also found that cytokine production from CD4+CD25- T cells was significantly reduced in CU patients (n = 9) compared to healthy donors (n = 11). Conclusions: Our data demonstrate that CD4+CD25+ and CD4+CD25- T cells in PBMCs exhibit defective functions in CU patients.
原文英語
頁(從 - 到)121-130
頁數10
期刊Journal of Dermatological Science
51
發行號2
DOIs
出版狀態已發佈 - 八月 2008

指紋

T-cells
Urticaria
T-Lymphocytes
Regulatory T-Lymphocytes
Blood
Transforming Growth Factors
Blood Cells
Cytokines
Flow cytometry
Peripheral Tolerance
Mitogens
Autoimmunity
Real-Time Polymerase Chain Reaction
Healthy Volunteers
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Tissue Donors

ASJC Scopus subject areas

  • Dermatology

引用此文

@article{dfc9adde661a44719cecacd768a45ec6,
title = "Defective functions of circulating CD4+CD25+ and CD4+CD25- T cells in patients with chronic ordinary urticaria",
abstract = "Background: Patients with chronic ordinary urticaria (CU) are divided into two groups: 30-50{\%} have chronic autoimmune urticaria, and the remainder have chronic idiopathic urticaria. CD4+CD25+ regulatory T (Treg) cells play critical roles in maintaining peripheral tolerance and preventing autoimmunity, but the characteristics of Treg cells have not yet been defined in CU. Objective: To identify whether CD4+ T cells play an important immunoregulatory role in the etiology of CU, we determined the frequencies and functions of circulating CD4+CD25+ and CD4+CD25- T cells in CU patients and healthy control subjects, with special focus on the characteristics of CD4+CD25+ T cells. Methods: Peripheral blood mononuclear cells (PBMCs) were obtained from CU and healthy controls in this study. The frequency of CD4+CD25+ T cells in PBMCs was detected by flow cytometry. The expression levels of forkhead box P3 (FOXP3) and transforming growth factor-β (TGF-β) in CD4+CD25+ T cells were detected by real-time PCR. Furthermore, the suppressive function of CD4+CD25+ T cells was analyzed. Additionally, the Th1/Th2 cytokine secretory profile in mitogen-stimulated CD4+CD25- T cells was measured by ELISA. Results: An increased frequency of CD4+CD25+ T cells was observed in CU patients (n = 19) compared to control subjects (n = 7). No significant difference was detected in the expression levels of FOXP3 or TGF-β between CU patients (n = 14) and control subjects (n = 7). Strikingly, the suppressive capacity of CD4+CD25+ Treg cells from 2 of 5 CU patients was partially defective. We also found that cytokine production from CD4+CD25- T cells was significantly reduced in CU patients (n = 9) compared to healthy donors (n = 11). Conclusions: Our data demonstrate that CD4+CD25+ and CD4+CD25- T cells in PBMCs exhibit defective functions in CU patients.",
keywords = "CD4CD25 regulatory T cells, CD4CD25 T cells, Chronic ordinary urticaria, Cytokines, FOXP3, Suppressive function",
author = "Chen, {Wu Charng} and Chiang, {Bor Luen} and Liu, {H. Eugene} and Leu, {Sy Jye} and Lee, {Yueh Lun}",
year = "2008",
month = "8",
doi = "10.1016/j.jdermsci.2008.02.012",
language = "English",
volume = "51",
pages = "121--130",
journal = "Journal of Dermatological Science",
issn = "0923-1811",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Defective functions of circulating CD4+CD25+ and CD4+CD25- T cells in patients with chronic ordinary urticaria

AU - Chen, Wu Charng

AU - Chiang, Bor Luen

AU - Liu, H. Eugene

AU - Leu, Sy Jye

AU - Lee, Yueh Lun

PY - 2008/8

Y1 - 2008/8

N2 - Background: Patients with chronic ordinary urticaria (CU) are divided into two groups: 30-50% have chronic autoimmune urticaria, and the remainder have chronic idiopathic urticaria. CD4+CD25+ regulatory T (Treg) cells play critical roles in maintaining peripheral tolerance and preventing autoimmunity, but the characteristics of Treg cells have not yet been defined in CU. Objective: To identify whether CD4+ T cells play an important immunoregulatory role in the etiology of CU, we determined the frequencies and functions of circulating CD4+CD25+ and CD4+CD25- T cells in CU patients and healthy control subjects, with special focus on the characteristics of CD4+CD25+ T cells. Methods: Peripheral blood mononuclear cells (PBMCs) were obtained from CU and healthy controls in this study. The frequency of CD4+CD25+ T cells in PBMCs was detected by flow cytometry. The expression levels of forkhead box P3 (FOXP3) and transforming growth factor-β (TGF-β) in CD4+CD25+ T cells were detected by real-time PCR. Furthermore, the suppressive function of CD4+CD25+ T cells was analyzed. Additionally, the Th1/Th2 cytokine secretory profile in mitogen-stimulated CD4+CD25- T cells was measured by ELISA. Results: An increased frequency of CD4+CD25+ T cells was observed in CU patients (n = 19) compared to control subjects (n = 7). No significant difference was detected in the expression levels of FOXP3 or TGF-β between CU patients (n = 14) and control subjects (n = 7). Strikingly, the suppressive capacity of CD4+CD25+ Treg cells from 2 of 5 CU patients was partially defective. We also found that cytokine production from CD4+CD25- T cells was significantly reduced in CU patients (n = 9) compared to healthy donors (n = 11). Conclusions: Our data demonstrate that CD4+CD25+ and CD4+CD25- T cells in PBMCs exhibit defective functions in CU patients.

AB - Background: Patients with chronic ordinary urticaria (CU) are divided into two groups: 30-50% have chronic autoimmune urticaria, and the remainder have chronic idiopathic urticaria. CD4+CD25+ regulatory T (Treg) cells play critical roles in maintaining peripheral tolerance and preventing autoimmunity, but the characteristics of Treg cells have not yet been defined in CU. Objective: To identify whether CD4+ T cells play an important immunoregulatory role in the etiology of CU, we determined the frequencies and functions of circulating CD4+CD25+ and CD4+CD25- T cells in CU patients and healthy control subjects, with special focus on the characteristics of CD4+CD25+ T cells. Methods: Peripheral blood mononuclear cells (PBMCs) were obtained from CU and healthy controls in this study. The frequency of CD4+CD25+ T cells in PBMCs was detected by flow cytometry. The expression levels of forkhead box P3 (FOXP3) and transforming growth factor-β (TGF-β) in CD4+CD25+ T cells were detected by real-time PCR. Furthermore, the suppressive function of CD4+CD25+ T cells was analyzed. Additionally, the Th1/Th2 cytokine secretory profile in mitogen-stimulated CD4+CD25- T cells was measured by ELISA. Results: An increased frequency of CD4+CD25+ T cells was observed in CU patients (n = 19) compared to control subjects (n = 7). No significant difference was detected in the expression levels of FOXP3 or TGF-β between CU patients (n = 14) and control subjects (n = 7). Strikingly, the suppressive capacity of CD4+CD25+ Treg cells from 2 of 5 CU patients was partially defective. We also found that cytokine production from CD4+CD25- T cells was significantly reduced in CU patients (n = 9) compared to healthy donors (n = 11). Conclusions: Our data demonstrate that CD4+CD25+ and CD4+CD25- T cells in PBMCs exhibit defective functions in CU patients.

KW - CD4CD25 regulatory T cells

KW - CD4CD25 T cells

KW - Chronic ordinary urticaria

KW - Cytokines

KW - FOXP3

KW - Suppressive function

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U2 - 10.1016/j.jdermsci.2008.02.012

DO - 10.1016/j.jdermsci.2008.02.012

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SP - 121

EP - 130

JO - Journal of Dermatological Science

JF - Journal of Dermatological Science

SN - 0923-1811

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