Decreased suppression of interleukin-6 after treatment with medroxyprogesterone acetate and danazol in endometrial stromal cells of women with adenomyosis

Jehn Hsiahn Yang, Mei Jou Chen, Ming Yih Wu, Yi Chun Chen, Yu Shih Yang, Hong Nerng Ho

研究成果: 雜誌貢獻文章同行評審

20 引文 斯高帕斯(Scopus)

摘要

Objective: To examine changes in local cytokine production in the eutopic endometrium of adenomyosis after treatment with medroxyprogesterone acetate (MPA) and danazol. Design: Prospective cohort study. Setting: Tertiary university hospital. Patient(s): Twenty-four women with adenomyosis (study group) and 22 women without adenomyosis (control group). Intervention(s): Eutopic endometrium was separated into single endometrial stromal cells (ESCs), and was cultured in vitro alone, with MPA (10-7 M), or with danazol (10-7 M) for 8 days. Main Outcome Measure(s): Messenger RNA expression of interleukin (IL)-6, IL-8, and tumor necrosis factor-α in ESCs, and their concentrations in the supernatants of ESCs. Result(s): A significant decrease of IL-6 concentration in the supernatant, measured by ELISA, was found with time of ESCs cultured with MPA and danazol in the control group, but not in adenomyosis. The IL-6 mRNA in ESCs determined by real-time quantitative polymerase chain, as well as its concentration in the supernatant, was much higher in adenomyosis than that in the control group after treatment with MPA and danazol for 8 days. Conclusion(s): Medroxyprogesterone acetate and danazol appeared to have a decreased effect on the suppression of IL-6 liberated by ESCs in adenomyosis. This aberrant secretion of IL-6 might play a role in the formation of ectopic endometrial implants in adenomyosis.
原文英語
頁(從 - 到)1459-1465
頁數7
期刊Fertility and Sterility
86
發行號5
DOIs
出版狀態已發佈 - 十一月 1 2006
對外發佈

ASJC Scopus subject areas

  • 生殖醫學
  • 婦產科

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