Decorin is a Zn2+ metalloprotein

Vivian W C Yang, Steven R. LaBrenz, Lawrence C. Rosenberg, David McQuillan, Magnus Höök

研究成果: 雜誌貢獻文章

29 引文 (Scopus)

摘要

Decorin is ubiquitously distributed in the extracellular matrix of mammals and a member of the proteoglycan family characterized by a core protein dominated by leucine-rich repeat motifs. We show here that decorin extracted from bovine tissues under denaturing conditions or produced in recombinant 'native' form by cultured mammalian cells has a high affinity for Zn2+ as demonstrated by equilibrium dialyses. The Zn2+-binding sites are localized to the N-terminal domain of the core protein that contains 4 Cys residues in a spacing reminiscent of a zinc finger. A recombinant 41-amino acid long peptide representing the N-terminal domain of decorin has full Zn2+ binding activity and binds two Zn2+ ions with an average K(D) of 3 x 10-7 M. Binding of Zn2+ to this peptide results in a change in secondary structure as shown by circular dichroism spectroscopy. Biglycan, a proteoglycan that is structurally closely related to decorin contains a similar high affinity Zn2+-binding segment, whereas the structurally more distantly related proteoglycans, epiphycan and osteoglycin, do not bind Zn2+ with high affinity.
原文英語
頁(從 - 到)12454-12460
頁數7
期刊Journal of Biological Chemistry
274
發行號18
DOIs
出版狀態已發佈 - 四月 30 1999
對外發佈Yes

指紋

Metalloproteins
Decorin
Proteoglycans
Biglycan
Circular dichroism spectroscopy
Peptides
Mammals
Dialysis
Zinc Fingers
Circular Dichroism
Extracellular Matrix
Zinc
Cultured Cells
Spectrum Analysis
Binding Sites
Cells
Ions
Tissue
Amino Acids
Proteins

ASJC Scopus subject areas

  • Biochemistry

引用此文

Yang, V. W. C., LaBrenz, S. R., Rosenberg, L. C., McQuillan, D., & Höök, M. (1999). Decorin is a Zn2+ metalloprotein. Journal of Biological Chemistry, 274(18), 12454-12460. https://doi.org/10.1074/jbc.274.18.12454

Decorin is a Zn2+ metalloprotein. / Yang, Vivian W C; LaBrenz, Steven R.; Rosenberg, Lawrence C.; McQuillan, David; Höök, Magnus.

於: Journal of Biological Chemistry, 卷 274, 編號 18, 30.04.1999, p. 12454-12460.

研究成果: 雜誌貢獻文章

Yang, VWC, LaBrenz, SR, Rosenberg, LC, McQuillan, D & Höök, M 1999, 'Decorin is a Zn2+ metalloprotein', Journal of Biological Chemistry, 卷 274, 編號 18, 頁 12454-12460. https://doi.org/10.1074/jbc.274.18.12454
Yang VWC, LaBrenz SR, Rosenberg LC, McQuillan D, Höök M. Decorin is a Zn2+ metalloprotein. Journal of Biological Chemistry. 1999 4月 30;274(18):12454-12460. https://doi.org/10.1074/jbc.274.18.12454
Yang, Vivian W C ; LaBrenz, Steven R. ; Rosenberg, Lawrence C. ; McQuillan, David ; Höök, Magnus. / Decorin is a Zn2+ metalloprotein. 於: Journal of Biological Chemistry. 1999 ; 卷 274, 編號 18. 頁 12454-12460.
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