Decorin binds fibrinogen in a Zn2+-dependent interaction

Tracey A. Dugan, Vivian W C Yang, David J. McQuillan, Magnus Höök

研究成果: 雜誌貢獻文章

29 引文 (Scopus)

摘要

We have previously shown that decorin, a member of the small leucine-rich proteoglycan family of extracellular matrix proteoglycans/glycoproteins is a Zn2+ metalloprotein at physiological Zn2+ concentrations (Yang, V. W-C., LaBrenz, S. R., Rosenberg, L. C., McQuillan, D., and Höök, M. (1999) J. Biol. Chem. 274, 12454-12460). We now report that the decorin proteoglycan binds fibrinogen in the presence of Zn2+. The fibrinogen-binding site is located in the N-terminal domain of the decorin core protein and a 45-amino acid peptide representing this domain binds to the fibrinogen D fragment with an apparent KD of 1.7 × 10-6 M, as determined from fluorescence polarization data. Furthermore, we show that Zn2+ promotes the self-association of decorin. The N-terminal domain of the core protein also mediates this activity. The results of solid-phase binding assays and gel filtration chromatography suggest that the N-terminal domain of decorin, when present at low micromolar concentrations, forms an oligomer in a Zn2+-dependent manner. Thus, Zn2+ appears to play a pivotal role in the interactions and biological function of decorin.
原文英語
頁(從 - 到)13655-13662
頁數8
期刊Journal of Biological Chemistry
278
發行號16
DOIs
出版狀態已發佈 - 四月 18 2003

指紋

Decorin
Fibrinogen
Proteoglycans
Metalloproteins
Fluorescence Polarization
Chromatography
Oligomers
Leucine
Gel Chromatography
Extracellular Matrix
Assays
Glycoproteins
Proteins
Gels
Fluorescence
Binding Sites
Association reactions
Polarization
Amino Acids
Peptides

ASJC Scopus subject areas

  • Biochemistry

引用此文

Decorin binds fibrinogen in a Zn2+-dependent interaction. / Dugan, Tracey A.; Yang, Vivian W C; McQuillan, David J.; Höök, Magnus.

於: Journal of Biological Chemistry, 卷 278, 編號 16, 18.04.2003, p. 13655-13662.

研究成果: 雜誌貢獻文章

Dugan, Tracey A. ; Yang, Vivian W C ; McQuillan, David J. ; Höök, Magnus. / Decorin binds fibrinogen in a Zn2+-dependent interaction. 於: Journal of Biological Chemistry. 2003 ; 卷 278, 編號 16. 頁 13655-13662.
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